High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. Fundamentally, chromatography is a technique used to separate the components contained in a sample. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures. The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. HPLC utilized the quality and quantity of both small molecules and polymeric species. High Performance Liquid Chromatography (HPLC) Analysis Services at Creative-Proteomics offers you a state-of-the-art liquid separations platform that includes standard HPLC with frequently used separation mechanisms.
NADP+ is the abbreviation of Nicotinamide adenine dinucleotide phosphate, and is is a cofactor used in anabolic reactions, such as lipid and nucleic acid synthesis. NADPH is the reduced form of NADP+. NADPH is produced in the last step of photosynthesis ferredoxin-NADP+ reductase in the photosynthetic organisms. In animals and other non-photosynthetic organisms, the major source of NADPH is the pentose phosphate pathway, which, generates NADPH and pentoses (5-carbon sugars) as well as Ribose 5-phosphate. In plants, NADP+ is used as reducing power for the biosynthetic reactions in the Calvin cycle to assimilate carbon dioxide. In animal, the regeneration of reduced glutathione, which is abbreviated as GSH in oxidation-reduction requires reducing equivalents provided by NADPH.
NADP Analysis Service at Creative Proteomics supports your research in NADP Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
NADP+/NADPH Analysis Service
Creative Proteomics's analytical scientists are highly experienced and knowledgeable in the application of HPLC analyses to a wide variety of industries ranging from Food & Beverage to Pharmaceuticals and much more. Experience, fast turnaround, clear concise written reports, and custom service are provided to help customers resolve your analytical and technical challenges.