Glycolysis pathway is a common metabolic pathway in both prokaryotic and eukaryotic cells. It takes place in the cytosol in eukaryotic cells. By converting glucose into two molecule of the three‐carbon compound pyruvate and generating two ATPs, glycolysis pathway generates the ATP needed for energy‐requiring processes and the pyruvate needed for the complete oxidation of glucose to carbon dioxide and water. For cells lack of mitochondria such as red blood cells, the glycolysis is the only energy source of ATP. For cells containing mitochondria, glycolysis serves as a preparatory pathway for the complete oxidation of glucose to generate larger amounts of ATP. This pathway often serves as a main energy source for cancer cell. Hence, glycolysis pathway is being extensively studied in cancer research, by analyzing the glycolytic enzymes expression level and monitoring the amount of metabolites.
Glycolysis pathway can be divided into three stages. Stage 1, phosphorylated by ATP, glucose converts to glucose 6-phosphate, and then to fructose 1,6-bisphosphate. In this stage, after a phosphorylation, an isomerization, and a second phosphorylation reaction, glucose is trapped and converts into a compound that can be easily cut into phosphorylated three-carbon units. Stage 2, the generated fructose 1,6-bisphosphate can be cleaved into two three-carbon fragments, which are readily convertible to each other. Stage 3, ATP is generated when the phosphorylated three-carbon units are oxidized to pyruvate.
LC–MS/MS platform enable simultaneous relative and absolute quantification of multiple metabolites in this pathway. The results are widely used for investigating the relative changes in metabolite concentrations, and potential biomarker discovery. By providing the exact molecular weights and retention time, LC–MS/MS techniques serves as a powerful analytical tool for identification and quantification of metabolites. Creative Proteomics has established sensitive, reliable, and accurate HPLC-MS/MS method for quantification of glycolysis pathway metabolites.
Feature and Advantage of glysolysis pathway metabolites analysis service:
- Cutting-edge facilities
- Reliable & Reproducible
- Highly sensitive: detect low abundance metabolites
- Comprehensive analysis of metabolites
Platform
- HPLC-MS/MS
Summary
- Identification and quantification of glycolysis pathway metabolites by HPLC-MS/MS
Sample Requirement
- Normal Volume: 200ul plasma; 100mg tissue; 2e7 cells
- Minimal Volume: 50uL plasma; 50mg tissue; 5e6 cells
Report
- A detailed technical report will be provided at the end of the whole project, including the experiment procedure, MS/MS instrument parameters
- Analytes are reported as uM or ug/mg (tissue), and CV's are generally<10%
- The name of the analytes, abbreviation, formula, molecular weight and CAS# would also be included in the report.
| Glycolysis Pathway Metabolites Quantified in This Service | ||
|---|---|---|
| 1,3 phosphate glycerate/2,3 phosphate glycerate | 3 phosphate glcerate/2 phosphate glycerate | Dihydroxyacetone phosphate |
| Fructose 6 phoshpate | Fructose 1 phoshpate | Fructose 1,6 bisphosphate |
| Glucose | Glucose 6 phosphate | Glyceraldehyde 3 phosphate |
| Phosphoenol pyruvate | Pyruvate | |
With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective glycolysis pathway metabolites targeted metabolomics services.