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Amino Acids and Derivatives Analysis Service

Amino acids are organic compounds containing basic amino and acidic carboxyl groups, which are the basic units of enzymes and proteins. As small biological molecules, amino acids play an important role in physiological functions and clinical diagnosis. In general, amino acids play the following roles in the body: (1) synthesize tissue proteins; (2) turn into ammonia-containing substances such as acids, hormones, antibodies, and creatine; (3) transform into carbohydrates and fats; and (4) oxidize into carbon dioxide and water and urea to produce energy.

There are 20 common amino acids according to the structure on the side chain R group. According to nutritional classification, it is divided into essential amino acids, semi-essential amino acids and conditionally essential amino acids. In addition, there are nearly a hundred kinds of derivatives of amino acids. Different amino acids and their derivatives have different nutritional values and physiological functions, and play different roles in the body. If the body is deficient in any one amino acid, it may lead to abnormal physiological functions,affect the normal progress of metabolism and finally lead to diseases. Arginine and citrulline are important for urea formation; insufficient intake of cystine can cause decreased insulin and increased blood sugar, etc. The presence of amino acids not only provides important raw materials for protein synthesis, but also provides the material basis for promoting growth, carrying out normal metabolism and maintaining life. If one of them is missing, the normal metabolism of the organism will be impaired, even leading to the occurrence of various diseases or the termination of life activities.

Our Amino Acids and Derivatives Analysis Service

After years of technical accumulation, Creative Proteomics has combined GC/MS and LC/MS/MS to achieve quantitative and qualitative analysis of more than 100 amino acids and their derivatives, including 8 essential amino acids and 2 semi-essential amino acids and conditionally essential amino acids, 10 non-essential amino acids, and other amino acids.

Quantitative Amino Acids List
1Glycine30L-Homoserine59Cysteinylglycine88N-Acetylaspartic acid
2L-Alanine31L-Thyronine60Guanidoacetic acid89N-Acetylcysteine
3L-Arginine321-Methyl-L-histidine61Homocitrulline90N-Acetylglutamic acid
5L-Aspartic acid342-aminobutyric acid63Argininosuccinic acid92N-Acetylhistidine
6L-Cysteine352-Aminoisobutyric acid64Glycylproline93N-Acetylisoleucine
7L-Cystine363-Aminobutyric acid65Glycyl-L-leucine94N-Acetylleucine
8L-Glutamic acid373-Aminoisobutyric acid66Carnosine95N-Acetylmethionine
9L-Glutamine38Gamma-Aminobutyric acid67Anserine96N-Acetylphenylalanine
10L-Histidine392,3-Diaminopropionic acid68Cystathionine97N-Acetylproline
11L-Isoleucine402-Aminoadipic acid69Glycyl-glycine98N-Acetyltryptophan
16L-Phenylalanine454-Acetamidobutyric acid74S-Adenosylhomocysteine103N6-Acetyllysine
17L-Proline464-Amino-3-hydroxybutyric acid75S-Adenosylmethionine104Ureidopropionic acid
18L-Serine474-Hydroxyphenylglycine76Sarcosine105Ureidosuccinic acid
20L-Tryptophan495-Aminopentanoic acid78Methylcysteine107Formylglycine
23L-Norleucine526-Aminocaproic acid81N-Acetylaspartylglutamic acid110N-Phenylacetylphenylalanine
24L-Norvaline53Asymmetric dimethylarginine82Phenylalanylphenylalanine111Tiglylglycine
25L-Pipecolic acid54Symmetric dimethylarginine83Dimethylglycine112Valylglycine
27L-Allo-isoleucine56Pyroglutamic acid85N2-Acetylornithine1143,5-Diiodo-L-tyrosine

Amino Acids and Derivatives Analysis Workflow

Amino Acids and Derivatives Analysis Workflow

Sample Requirements

Animal and clinical tissue samples: > 5-10mg

Blood samples (serum, plasma and whole blood):>10uL

Urine sample:>10uL

Fecal and intestinal contents:>10mg

Body fluid samples (cerebrospinal fluid and saliva, etc.): >5-10μL

Plant tissue samples (roots, stems, leaves flowers and fruits, etc.): >10mg

Cells and microorganisms:>1*10^5cells

Culture medium and fermentation broth:>10μL



Waters UPLC-AB Sciex MS/MS

Thermo Scientific Orbitrap MS

Agilent GC-MS


Phenome and physiological function studies.

Early clinical prediction, diagnosis and treatment studies.

Pathology studies.

Natural drugs and pharmacological research.

Food science, safety and nutrition research.

Livestock and agroforestry research.

Botanical and environmental research.

Toxicological research.

* For Research Use Only. Not for use in diagnostic procedures.
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