Nicotinamide adenine dinucleotide (NAD) is a coenzyme, which can be found in all living cells. NAD consists of two nucleotides joined through their phosphate groups and it is a dinucleotide, in which the one nucleotide contains an adenine base and the other nicotinamide. There are two different NAD forms exist naturally, the one is an oxidized form, which is abbreviated as NAD+ and the other is a reduced form, which is abbreviated as NADH.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of NAD+ levels in various samples, including Tissue, Plant, Bacterials, Cells and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of NAD+/NADH (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of NAD+ measurement, which enables us to analyze of NAD+ levels in vitro and in vivo.
Nicotinamide adenine dinucleotide participates in redox reactions, which can carry electrons from one reaction to another In metabolism. Therefore, nicotinamide adenine dinucleotide is a coenzyme, can be found in two forms in cells, one is an oxidizing agent-NAD+, which can accepts electrons from other molecules and becomes reduced form, which is called NADH. NADH then can be used as a reducing agent to donate electrons and these electron transfer reactions are the main function of NAD. In addition, nicotinamide adenine dinucleotide is also involved in other cellular processes, the most significant one is in the process of posttranslational modifications, NAD can be used as a substrate of enzymes that add or remove chemical groups from proteins. Due to these important biochemical functions, the enzymes involved in NAD metabolism reactions are considered as drug targets for drug discovery and development.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Nicotinamide adenine dinucleotide (NAD+) Analysis Service at Creative Proteomics supports your research in Nicotinamide adenine dinucleotide (NAD+) Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Tissue, Plant, Bacterials, Cells and more
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of NAD+/NADH (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of NAD+ measurement, which enables us to analyze of NAD+ levels in vitro and in vivo.
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