Mannose is a sugar monomer and belongs to the aldohexose. Mannose is a C-2 epimer of glucose and the chemical molecular formula is C6H12O6. Mannose commonly exists as two different sized rings, the one is named pyranose with six-membered and the other is furanose with five-membered.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Mannose levels in various samples, including Food, Beverage and more. High-Performance Liquid Chromatography (HPLC) using a differential refractive index detector (RID) for the determination of Mannose levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Mannose measurement, which enables us to analyze of Mannose levels in vitro and in vivo.
Mannose presents in the form of N-linked glycosylation of proteins in abundant amount and C- mannosylation can be found in collagen-like regions in a large amount. The digestion of many polysaccharides and glycoproteins results the increase of the amount of Mannose, and then is phosphorylated by hexokinase to produce Mannose-6-phosphate, which is can be converted to fructose-6-phosphate derived by the enzyme named as phosphoMannose isomerase. In the following biochemical reaction, the fructose-6-phosphate can enter the glycolytic pathway, in which can be converted into pyruvate, or converted to glucose-6-phosphate by the gluconeogenic pathway of hepatocytes. Mannose plays an important in human metabolism, especially in the proteins glycosylation, which is the reaction in which a glycosyl donor is attached to a hydroxyl or other functional group of another molecule. D-Mannose powder may help to prevent recurrent urinary tract infections, which is an infection that affects part of the urinary tract.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Mannose Analysis Service at Creative Proteomics supports your research in Mannose Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Sample Type
Food, Beverage and more
Method
High-Performance Liquid Chromatography (HPLC) using a differential refractive index detector (RID) for the determination of Mannose levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Mannose measurement, which enables us to analyze of Mannose levels in vitro and in vivo.
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