Coenzyme Q10, also known as ubiquinone, ubidecarenone, coenzyme Q, and is abbreviated to CoQ10. It is a 1,4-benzoquinone, where Q refers to the quinone chemical group and 10 refers to the number of isoprenyl chemical subunits in its tail. CoQ10 is a coenzyme which is ubiquitous in the bodies of most animals, especially on the membranes of many organelles.

Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Coenzyme Q10 levels in various samples, including Yeast, Tissue, Plant, and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of CoQl0 (275 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Coenzyme Q10 measurement, which enables us to analyze of Coenzyme Q10 levels in vitro and in vivo.

CoQ10 is well defined as a crucial component of the electron transport chain and participates in aerobic cellular respiration in mitochondria which converts biochemical energy from nutrients into adenosine triphosphate (ATP) and about ninety-five percent of the human body’s energy is generated this way. CoQ10 functions as an electron carrier from enzyme complex I and enzyme complex II to complex III in aerobic cellular respiration. This is very important in the process, since no other molecule can perform this function. Thus, the primary function of CoQ10 in every cell of the body is in generating energy. CoQ10 continuously goes through an oxidation–reduction cycle as an energy carrier and it becomes reduced when it accepts electrons, as it gives up electrons, it becomes oxidized. CoQ10 can act as an antioxidant because the CoQ10 molecule can give up one or both electrons quite easily in its reduced form.

The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.

Coenzyme Q10 Analysis Service at Creative Proteomics supports your research in Coenzyme Q10 Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.

Sample Type
Yeast, Tissue, Plant, and more

Method
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of CoQl0 (275 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Coenzyme Q10 measurement, which enables us to analyze of Coenzyme Q10 levels in vitro and in vivo.

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