The Thermo Scientific Q Exactive hybrid quadrupole-Orbitrap mass spectrometer introduce the concept of Quanfirmation, which means to routinely and confidently identify, quantify, and confirm in a single analysis using a single mass spectrometer. The Thermo Scientific Q Exactive Plus takes Quanfirmation to a new level.
Figure 1. The Thermo Scientific Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer
The Structure of the Q Exactive Plus Mass Spectrometer
Figure 2. Schematic of the Q Exactive Plus mass spectrometer
Like Q Exactive mass spectrometer, the Q Exactive Plus consists of six main components, including the ion source, an injection flatapole with mass resolving capabilities, quadrupole mass filter for precursor ion selection, curved linear trap (C-trap) for short selection, collision cell, and Orbitrap analyzer for Fourier transform mass analysis.
The injection flatapole acts as an ion focusing device and also an ion pre-filtering device. To focus the ions along the axis of the lens, an RF voltage is applied to the electrodes. In addition, a DC voltage is applied to the injection flatapole. In positive ion mode, this voltage creates a negative potential difference with respect to the S-lens exit lens. While in negative ion mode, this voltage difference is inverted. In ion pre-filtering mode, additional DC voltages (of opposite sign but equal magnitude) are applied to opposite sets of electrodes to coarsely filter ions falling outside of a wide window around the much narrower window of ions passed by the quadrupole. For quadrupole mass filter, the Q Exactive Plus MS uses advanced quadrupole technology (AQT), which comprises a hyperbolic segmented quadrupole for higher transmission efficiency and optimized transmission window shape.
In Q Exactive Plus mass spectrometer, after samples are introduced into the API source, the injection flatapole transmits ions into the quadrupole. In addition, the injection flatapole also work as coarse pre-filtering of ions according to their mass-to-charge (m/z) ratios. The quadrupole rod can transmit and filter the ions according to m/z ratios. And then ions are transferred into the C-trap, where the ions are accumulated and their energy is dampened with a bath gas. The ions are then injected into the Orbitrap analyzer to obtain mass spectra. Besides, to conduct MS/MS experiment or all-ion fragmentation (AIF), the ions are passed through the C-Trap into the HCD cell for fragmentation. After fragmentation, the ions are transferred back into the C-Trap and injected into the Orbitrap analyzer for detection.
Features of Q Exactive Plus Mass Spectrometer
Figure 3. Features of Q Exactive Plus Mass Spectrometer
Applications of Q Exactive Plus Mass Spectrometer
The Q Exactive Plus mass spectrometer accelerates a wide range of applications.
The Q Exactive Plus mass spectrometer combines the quadrupole precursor selection with HR/AM Orbitrap to provide identification and quantitation of more compounds with greater confidence. At Creative Proteomics, we can provide the reliable services to meet a variety of requirements by using the Thermo Scientific Q Exactive Plus hybrid quadrupole Orbitrap mass spectrometer.
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