Spermine is a polyamine, which is involved in cellular metabolism and are present in virtually all living cells and a wide variety of polyamines are formed in nature according to the species. The Spermine was as first reported as a component of human seminal plasma by Leeuwenhoek in 1678. The name spermin was first used by t Ladenburg and Abel in 1888, which are chemists from German. The correct structure of Spermine was established in1926, by Dudley, Rosenheim, Starling and Wrede. Spermine is found as a polycation at physiological pH. Spermine is associated with nucleic acids and the function is considered to stabilize helical structure, in particular, in viruses. The precursor for synthesis of Spermine is ornithine, which can be found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Spermine levels in various samples, including Serum, Tissue and more. Biogenic amines of Spermine in a lot of biological samples were detected by High-Performance Liquid Chromatography (HPLC), using precolumn derivatization with DNS-Cl. This Methodology provides accurate, reliable, and reproducible results of Spermine measurement, which enables us to analyze of Spermine levels in vitro and in vivo.
Mammal cells can produce Spermine, spermidine, and their precursor, the diamine putrescine, and these polyamines play critical roles in many cellular biochemical processes, for example, regulation of transcription and translation, control of the activity of ion channels, modulation of kinase activities, effects on the cell cycle, protection from oxidative damage, the maintenance of membrane structure/function, and contributing to nucleic acid structure and stability and so on.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Spermine Analysis Service at Creative Proteomics supports your research in Spermine Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Serum, Tissue and more
Biogenic amines of Spermine in a lot of biological samples were detected by High-Performance Liquid Chromatography (HPLC), using precolumn derivatization with DNS-Cl. This Methodology provides accurate, reliable, and reproducible results of Spermine measurement, which enables us to analyze of Spermine levels in vitro and in vivo.
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