Spermidine is a polyamine compound and the chemical formulation is (C7H19N3), which can be found in ribosomes and living tissues. It was originally isolated from semen. Spermidine is a kind of polyamines and is polycationic aliphatic amines. Spermidine is multifunctional and has various metabolic functions within organisms. The formation of Spermidine from putrescine is catalyzed by Spermidine synthase. Spermidine is a longevity agent in mammals due to Spermidine is involved in various mechanisms of biological reaction.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Spermidine levels in various samples, including Serum, Tissue and more. Biogenic amines of Spermidine in a lot of biological samples were detected by High-Performance Liquid Chromatography (HPLC), using precolumn derivatization with DNS-Cl. This Methodology provides accurate, reliable, and reproducible results of Spermidine measurement, which enables us to analyze of Spermidine levels in vitro and in vivo.
Although the various mechanisms of action of Spermidine in mammals, the function of Spermidine is just beginning to be understood. Spermidine can synchronizes many biological processes (such as Ca2+, Na+, K+ -ATPase) and then maintaining membrane potential and controlling intracellular pH and volume. Spermidine regulates Ca2+ influx through glutamatergic N-methyl-d-aspartate receptor (NMDA receptor), which has been reported associated with nitric oxide synthase (NOS) and cGMP/PKG pathway activation and a activity decrease of Na+,K+-ATPase in cerebral cortex synaptosomes.
Spermidine is a longevity agent in mammals due to the important role in gene regulation and the biological mechanism is not clearly. Spermidine is also known to regulate the plant growth, which can regulate the process of transcribing RNA, inhibition the production of NOS. In addition, Spermidine is a precursor to other polyamines, such as spermine and thermospermine, some of which have important role in the tolerance against drought and salinity in plants. Spermidine also is reported can encourage hair shaft elongation and lengthen hair growth. Spermidine has also been found to upregulate the expression level of the keratins K15 and K19, which are associated with epithelial stem cell, and regulate the K15 promoter activity in a dose dependently manner.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Spermidine Analysis Service at Creative Proteomics supports your research in Spermidine Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Serum, Tissue and more
Biogenic amines of Spermidine in a lot of biological samples were detected by High-Performance Liquid Chromatography (HPLC), using precolumn derivatization with DNS-Cl. This Methodology provides accurate, reliable, and reproducible results of Spermidine measurement, which enables us to analyze of Spermidine levels in vitro and in vivo.
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