Spermidine Analysis Service


Spermidine Analysis Service

Spermidine is a polyamine compound and the chemical formulation  is (C7H19N3), which can be found in ribosomes and living tissues. It was  originally isolated from semen. Spermidine is a kind of polyamines and is polycationic aliphatic amines. Spermidine is multifunctional and has  various metabolic functions within organisms. The formation of Spermidine from putrescine is catalyzed  by Spermidine synthase. Spermidine is a longevity agent in  mammals due to Spermidine is  involved in various mechanisms of biological reaction.

Scientists at Creative Proteomics utilize a highly quantitative method with  high-performance liquid chromatography (HPLC)  for the determination of Spermidine levels in various samples, including Serum,  Tissue and more. Biogenic amines of Spermidine in a lot of biological samples  were detected by High-Performance Liquid Chromatography (HPLC), using precolumn derivatization with DNS-Cl. This Methodology  provides accurate, reliable, and reproducible results of Spermidine  measurement, which enables us to analyze of Spermidine levels in vitro and in  vivo.

Although the various mechanisms of action of Spermidine in mammals, the function of Spermidine is just beginning to be  understood. Spermidine can  synchronizes many biological processes (such as Ca2+, Na+, K+ -ATPase) and then  maintaining membrane potential and controlling intracellular pH and volume. Spermidine regulates Ca2+ influx  through glutamatergic N-methyl-d-aspartate receptor (NMDA receptor), which has  been reported associated with nitric oxide synthase (NOS) and cGMP/PKG pathway  activation and a activity decrease of Na+,K+-ATPase in cerebral cortex  synaptosomes.

Spermidine is a  longevity agent in mammals due to the important role in gene regulation and the  biological mechanism is not clearly. Spermidine is also known to regulate the plant growth, which can regulate the process of  transcribing RNA, inhibition the production of NOS. In addition, Spermidine is a precursor to other  polyamines, such as spermine and thermospermine, some of which have important  role in the tolerance against drought and salinity in plants. Spermidine also is reported can  encourage hair shaft elongation and lengthen hair growth. Spermidine has also been found to upregulate the expression level  of the keratins K15 and K19, which are associated with epithelial stem cell,  and regulate the K15 promoter activity in a dose dependently manner.

The Russian-Polish botanist M. Tswett is  generally recognized as the first person to establish the principles of  chromatography. In a paper he presented in 1906, Tswett described how he filled  a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of  chlorophyll to flow through the chalk, separated the chlorophyll into layers of  different colors. He called this technique “chromatography”. Fundamentally,  chromatography is a technique used to separate the components contained in a  sample. High Performance Liquid  Chromatography (HPLC) is a method able to separate non-volatile, thermally  unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide  application range and quantitative accuracy, is regarded as an indispensable  analytical technique, particularly in the field of organic chemistry. It is  also widely used as a preparation technique for the isolation and purification  of target components contained in mixtures.

Spermidine Analysis  Service at Creative Proteomics supports your research in Spermidine Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.

Sample  Type
Serum, Tissue and more

Method
Biogenic  amines of Spermidine in a lot of  biological samples were detected by High-Performance Liquid Chromatography (HPLC), using precolumn derivatization  with DNS-Cl. This Methodology provides accurate, reliable, and reproducible  results of Spermidine measurement,  which enables us to analyze of Spermidine levels in vitro and in vivo.

Send us your samples, you will get all information that you  need!


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