Putrescine, is also known as tetramethylenediamine, was first described in 1885 by the Berlin physician Ludwig Brieger. Putrescine is a foul-smelling organic chemical compound with the chemical formula is NH2(CH2)4NH2 (1,4-diaminobutane or butanediamine) which is related to cadaverine. They are produced by the breakdown of amino acids in living and dead organisms and both are toxic in high doses. In rats it has a low acute oral toxicity of 2000 mg/kg body weight, with no-observed-adverse-effect level of 180 mg/kg body weight. The two compounds are mainly responsible for the foul odor of putrefying flesh, but also contribute to the odor of the processes such as bad breath and bacterial vaginosis. They are also found in semen and some microalgae, which are accompanied by the related molecules like spermine and spermidine.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Putrescine levels in various samples, including Serum, Tissue and more. Biogenic amines of Putrescine in a lot of biological samples were detected by High-Performance Liquid Chromatography (HPLC), using precolumn derivatization with DNS-Cl. This Methodology provides accurate, reliable, and reproducible results of Putrescine measurement, which enables us to analyze of Putrescine levels in vitro and in vivo.
Putrescine attacks decarboxylated S-adenosyl methionine and then can be converted to spermidine, which in turn attacks another decarboxylated S-adenosyl methionine and is converted to spermine in biochemical reaction. Putrescine is synthesized in small amount by healthy living cells through the action of ornithine decarboxylase. Putrescine can be synthesized through two different biological pathways, both starting from arginine. In one biological pathway, arginine is converted into agmatine, through a reaction catalyzed by the enzyme called arginine decarboxylase (ADC); then agmatine is transformed into N-carbamoylPutrescine by agmatine imino hydroxylase (AIH). N-carbamoylPutrescine is converted into Putrescine in the finally step. In the other pathway, arginine is converted into ornithine firstly, which then is converted into Putrescine by the enzyme called ornithine decarboxylase (ODC).
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Putrescine Analysis Service at Creative Proteomics supports your research in Putrescine Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Serum, Tissue and more
Biogenic amines of Putrescine in a lot of biological samples were detected by High-Performance Liquid Chromatography (HPLC), using precolumn derivatization with DNS-Cl. This Methodology provides accurate, reliable, and reproducible results of Putrescine measurement, which enables us to analyze of Putrescine levels in vitro and in vivo.
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