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Putrescine Analysis Service

Putrescine Analysis Service

Putrescine, is also known as tetramethylenediamine, was first  described in 1885 by the Berlin physician Ludwig Brieger. Putrescine is a foul-smelling organic chemical compound with the chemical formula is NH2(CH2)4NH2  (1,4-diaminobutane or butanediamine) which is related to cadaverine. They are  produced by the breakdown of amino acids in living and dead organisms and both  are toxic in high doses. In rats it has a low acute oral toxicity of 2000 mg/kg  body weight, with no-observed-adverse-effect level of 180 mg/kg body weight.  The two compounds are mainly responsible for the foul odor of putrefying flesh,  but also contribute to the odor of the processes such as bad breath and  bacterial vaginosis. They are also found in semen and some microalgae, which  are accompanied by the related molecules like spermine and spermidine.

Scientists at Creative Proteomics utilize a highly quantitative method with  high-performance liquid chromatography (HPLC)  for the determination of Putrescine levels in various samples, including Serum,  Tissue and more. Biogenic amines of Putrescine in a lot of biological samples  were detected by High-Performance Liquid Chromatography (HPLC), using precolumn derivatization with DNS-Cl. This Methodology  provides accurate, reliable, and reproducible results of Putrescine  measurement, which enables us to analyze of Putrescine levels in vitro and in  vivo.

Putrescine attacks decarboxylated S-adenosyl methionine and then  can be converted to spermidine, which in turn attacks another decarboxylated  S-adenosyl methionine and is converted to spermine in biochemical reaction. Putrescine is synthesized in small  amount by healthy living cells through the action of ornithine decarboxylase. Putrescine can be synthesized through  two different biological pathways, both starting from arginine. In one  biological pathway, arginine is converted into agmatine, through a reaction  catalyzed by the enzyme called arginine decarboxylase (ADC); then agmatine is  transformed into N-carbamoylPutrescine by  agmatine imino hydroxylase (AIH). N-carbamoylPutrescine is converted into Putrescine in the finally step. In the other pathway, arginine is converted into  ornithine firstly, which then is converted into Putrescine by the enzyme called ornithine decarboxylase (ODC).

The Russian-Polish botanist M. Tswett is  generally recognized as the first person to establish the principles of  chromatography. In a paper he presented in 1906, Tswett described how he filled  a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of  chlorophyll to flow through the chalk, separated the chlorophyll into layers of  different colors. He called this technique “chromatography”. Fundamentally,  chromatography is a technique used to separate the components contained in a  sample. High Performance Liquid  Chromatography (HPLC) is a method able to separate non-volatile, thermally  unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide  application range and quantitative accuracy, is regarded as an indispensable  analytical technique, particularly in the field of organic chemistry. It is  also widely used as a preparation technique for the isolation and purification  of target components contained in mixtures.

Putrescine Analysis  Service at Creative Proteomics supports your research in Putrescine Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.

Sample  Type
Serum,  Tissue and more

Biogenic amines of Putrescine in a lot of biological  samples were detected by High-Performance Liquid Chromatography (HPLC), using precolumn derivatization  with DNS-Cl. This Methodology provides accurate, reliable, and reproducible  results of Putrescine measurement,  which enables us to analyze of Putrescine levels in vitro and in vivo.

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* For Research Use Only. Not for use in diagnostic procedures.
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