Malic acid is an organic compound and the chemical molecular formulation is C4H6O5. Malic acid is a dicarboxylic acid, which is an organic compound containing two carboxyl functional groups (-COOH). Malic acid can be made by all living organisms and is found in many fruits, for example, apples, tartaric acid occurs in grapes and other citrus fruits. Malic acid was first isolated from apple juice by Carl Wilhelm Scheele, who is a Swedish Pomeranian and pharmaceutical chemist in 1785. Malic acid has two stereoisomeric forms, just as the L- and D-enantiomers and L-Malic acid is the naturally occurring form, whereas a mixture of L- and D-Malic acid is produced synthetically.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Malic Acid levels in various samples, including Plant. High-Performance Liquid Chromatography (HPLC) using a differential refractive index detector (RID) for the determination of Malic acid levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Malic acid measurement, which enables us to analyze of Malic acid levels in vitro and in vivo.
Malate plays an important role in biochemistry and the malate anion is an intermediate in the citric acid cycle, which is a series of chemical reactions used by all aerobic organisms to generate energy in biochemistry. Malate is a source of CO2 in the Calvin cycle, which is a serial of chemical reactions that convert carbon dioxide and other compounds into glucose. Malate can also be formed from pyruvate via anaplerotic reactions, which are chemical reactions that form intermediates of a metabolic pathway.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Malic acid Analysis Service at Creative Proteomics supports your research in Malic acid Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Sample Type
Plant
Method
High-Performance Liquid Chromatography (HPLC) using a differential refractive index detector (RID) for the determination of Malic acid levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Malic acid measurement, which enables us to analyze of Malic acid levels in vitro and in vivo.
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