Ferulic Acid Analysis Service

Creative Proteomics provides precise ferulic acid analysis using advanced HPLC, LC-MS, and GC-MS techniques to ensure accurate quantification and characterization in food, pharmaceuticals, cosmetics, and botanical extracts. Our services help businesses verify product composition, meet regulatory standards, and enhance research on antioxidant properties, ensuring quality, safety, and efficacy.

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What are Ferulic Acid?

Ferulic acid (4-hydroxy-3-methoxycinnamic acid) is a phenolic compound widely distributed in plant cell walls, particularly in grains, fruits, and vegetables. It exhibits potent antioxidant, anti-inflammatory, and UV-protective properties, making it valuable in pharmaceuticals, nutraceuticals, and cosmetics. Its ability to scavenge free radicals, inhibit lipid peroxidation, and modulate enzymes like glutathione transferase underpins its therapeutic potential in treating cardiovascular diseases, cancer, and neurodegenerative disorders.

What Projects Does Creative Proteomics Offer?

Creative Proteomics offers precise Ferulic Acid analysis services using advanced techniques to meet the needs of various industries, including healthcare, pharmaceuticals, food production, and cosmetics.

Ferulic Acid Quantification: We provide accurate quantification of ferulic acid in a variety of sample types, including food, plant extracts, and pharmaceuticals.
Identification and Characterization: Our services include identifying and characterizing ferulic acid in complex matrices, such as herbal formulations and botanical extracts.
Separation and Purification: We specialize in isolating and purifying ferulic acid from complex biological matrices, ensuring high purity for further research or product development.
Toxicological and Pharmacokinetic Studies: We offer toxicity screening and pharmacokinetic profiling of ferulic acid for its potential use as an active pharmaceutical ingredient or dietary supplement.
Customized Analysis and Consultation: Our experts can design tailored methodologies to meet specific analytical needs for your research or product development.

Detectable Ferulic Acid Derivatives

Our platforms identify over 50 ferulic acid conjugates with industry-leading accuracy:

Free Ferulic Acid: Plasma, plant extracts (LOQ: 0.05 µg/mL via HPLC).
Ferulic Acid Glucuronide: Urine, cell lysates (LOQ: 0.02 µg/mL via LC-MS).
5-5' Diferulic Acid: Lignocellulosic biomass (LOQ: 0.2 µg/mL via HPLC).
Ethyl Ferulate: Cosmetic emulsions (LOQ: 0.03 µg/mL via GC-MS)

Methods for Ferulic Acid Detection

High-Performance Liquid Chromatography (HPLC)

Instrument: Agilent 1260 Infinity II with C18 reverse-phase column.

Detection: Diode Array Detector (DAD) at 323 nm, validated for dissolution testing (USP Apparatus II) .

Liquid Chromatography-Mass Spectrometry (LC-MS)

Instrument: Thermo Scientific Q Exactive HF-X Orbitrap.

Resolution: 140,000 FWHM for precise quantification of glucuronides and sulfates.

Gas Chromatography-Mass Spectrometry (GC-MS)

Instrument: Agilent 7890B with DB-5MS column.

Derivatization: BSTFA/TMCS for volatile derivatives

Agilent 1260 Infinity II HPLC (Fig from Agilent)

Thermo Fisher Scientific Q Exactive™ HF-X Hybrid Quadrupole-Orbitrap™ Mass Spectrometer

Q Exactive™ HF-X Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (Fig from Thermo Fisher)

Agilent 7890B Gas chromatography system (Fig from Agilent)

Advantages of Our Ferulic Acid Detection

Ultra-High Sensitivity-Detection limits as low as 0.01 µg/mL for trace-level quantification.
Multi-Matrix Compatibility-Validated for plant tissues, pharmaceuticals, cosmetics, serum, and food products.
Rapid Turnaround-Standard analysis completed within 14–21 business days; expedited options available.
Unmatched Precision-≤2% RSD (relative standard deviation) in inter-day reproducibility tests.
Expertise-Driven-Analysis supervised by PhD-level scientists with 20+ years in phytochemical analytics.
Custom Method Development-Tailored protocols for novel ferulic acid derivatives or complex matrices.

Sample Requirements for Ferulic Acid Analysis

Sample Type	Recommended Sample Volume	Preparation Guidelines
Food Samples	0.5 – 10 g	Homogenize solid samples; liquid samples should be filtered or centrifuged.
Plant Extracts	1 – 5 mL	Concentrated extracts preferred; ensure uniformity for accurate quantification.
Pharmaceutical Formulations	1 – 5 g	Tablets, capsules, or powders should be finely ground or dissolved before analysis.
Botanical Extracts	1 – 5 mL	Concentrated extracts or standardized preparations work best for consistency.
Biological Samples (Blood, Plasma, Urine)	1 – 5 mL	Ensure samples are collected in appropriate tubes (e.g., EDTA or heparin for blood) and stored at -20°C or lower.
Tissues (Plant or Animal)	0.5 – 1 g	Homogenize tissue samples and store at -80°C before sending for analysis.

Applications of Ferulic Acid Assay

Food Industry

Measure antioxidant levels in grains, fruits, vegetables, and processed food products.

Pharmaceutical Industry

Quantify ferulic acid for use in drug formulations, ensuring proper dosing and consistency.

Cosmetic Industry

Assess ferulic acid concentrations in skincare products for anti-aging and UV protection benefits.

Nutraceuticals

Standardize dietary supplements containing ferulic acid to meet label claims.

Agricultural Research

Study ferulic acid's role in plant defense mechanisms and stress resistance.

Botanical Research

Quantify ferulic acid in plant extracts for bioactive compound analysis and product development.

Demo Result of Targeted Metabolomics Service

Figures come from (Li, Y.et.al, Sci Rep,2023)

Principal Component Analysis (PCA) chart showing the distribution of samples across principal components

Quickly show the distribution of samples across principal components.

Orthogonal Partial Least Squares Method-Discriminant Analysis (PLS-DA) point cloud diagram illustrating the separation of sample groups in a multidimensional space

OPLS-DA point cloud diagram

Illustrate the separation of sample groups in a multidimensional space.

Plot of multiplicative change volcanoes

Volcano plot depicting multiplicative changes in metabolite levels.

Dendrogram representing hierarchical clustering of samples.

Sample Hierarchical Clustering

Each column in the figure represents a sample

FAQ of Ferulic Acid Analysis

How accurate are your detection methods for low-concentration samples?

Our LC-MS/MS systems achieve 0.01 µg/mL sensitivity, validated for trace-level analysis in complex matrices like serum and plant extracts, with ≤5% CV (coefficient of variation).

Can you analyze ferulic acid in both raw materials and finished products?

Yes. We support end-to-end testing, from raw plant biomass (e.g., rice bran) to final formulations (tablets, emulsions), adhering to USP/EP monographs for purity and stability.

What sample preparation is required? Do you offer extraction services?

Minimal prep needed: freeze biological samples, avoid light exposure for photosensitive compounds. We provide freeze-drying, SPE extraction, and derivatization as add-on services.

Learn about other Q&A.

Ferulic Acid Analysis Case Study

Article

Identification and Quantification of Phenolic Acids in Mushrooms

Publications

Here are some publications in proteomics research from our clients:

Quantification of choline in serum and plasma using a clinical nuclear magnetic resonance analyzer. 2022. https://doi.org/10.1016/j.cca.2021.11.031
Quantifying forms and functions of intestinal bile acid pools in mice. 2024. https://doi.org/10.1101/2024.02.16.580658
Ketone bodies are mildly elevated in subjects with type 2 diabetes mellitus and are inversely associated with insulin resistance as measured by the lipoprotein insulin resistance index. 2020. https://doi.org/10.3390/jcm9020321
Sarcosine Is Uniquely Modulated by Aging and Dietary Restriction in Rodents and Humans. 2018. https://doi.org/10.1016/j.celrep.2018.09.065
Effect of arabinogalactan on the gut microbiome: A randomized, double-blind, placebo-controlled, crossover trial in healthy adults. 2021. https://doi.org/10.1016/j.nut.2021.111273

More Publications

Metabolomics Sample Submission Guidelines

Download our Metabolomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted metabolomics.

Download

Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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