Cyanidin-3-O-glucoside (Chrysanthemin) Analysis Service


Cyanidin-3-O-glucoside (Chrysanthemin)Analysis Service

Chrysanthemin is a kind of anthocyanin and it is the 3-glucoside of  cyanidin. Chrysanthemin can be found  in the roselle plant (Hibiscus sabdariffa, Malvaceae), different Japanese  angiosperms and Rhaponticum (Asteraceae), the fruits of the smooth arrowwood  (Viburnum dentatum, Caprifoliaceae) appear blue and one of the major pigments  is cyanidin 3-glucoside, although the total mixture is very complex. Chrysanthemin is also can be found in  blackcurrant pomace, European  elderberry, red raspberries, soybean seed coats, victoria plum, peach,  lychee and açaí. Chrysanthemin is  also can be detected in the red oranges and black rice.

Scientists at Creative Proteomics utilize a highly quantitative method with  high-performance liquid chromatography (HPLC)  for the determination of Cyanidin-3-O-glucoside (Chrysanthemin) levels in  various samples, including Plant, Tissue and more. High-Performance Liquid  Chromatography (HPLC) with UV  detection is used for the determination of C3G (530 nm) levels in a lot of  biological samples. This Methodology provides accurate, reliable, and  reproducible results of Cyanidin-3-O-glucoside (Chrysanthemin) measurement,  which enables us to analyze of C3G levels in vitro and in vivo.
 Chrysanthemin is the major anthocyanin of purple corn (Zea mays),  which is approved in Japan and listed in the "Existing Food Additive  List" as purple corn color. By the mean of metabolic genetic  engineering,the biosynthesis of cyanidin 3-O-glucoside in Escherichia coli was  reported in many research papers. A glycosyltransferase, called as UGT79B1 is  involved in the anthocyanin biosynthetic pathway in Arabidopsis thaliana,  UGT79B1 protein converts cyanidin 3-O-glucoside to cyanidin 3-O-xylosyl (1→2)  glucoside.

The Russian-Polish botanist M. Tswett is  generally recognized as the first person to establish the principles of  chromatography. In a paper he presented in 1906, Tswett described how he filled  a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of  chlorophyll to flow through the chalk, separated the chlorophyll into layers of  different colors. He called this technique “chromatography”. Fundamentally,  chromatography is a technique used to separate the components contained in a  sample. High Performance Liquid  Chromatography (HPLC) is a method able to separate non-volatile, thermally  unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide  application range and quantitative accuracy, is regarded as an indispensable  analytical technique, particularly in the field of organic chemistry. It is  also widely used as a preparation technique for the isolation and purification  of target components contained in mixtures.

Cyanidin-3-O-glucoside (Chrysanthemin) Analysis Service at Creative  Proteomics supports your research in Cyanidin-3-O-glucoside (Chrysanthemin) Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.

Sample  Type
Plant,  Tissue and more

Method
High-Performance  Liquid Chromatography (HPLC) with UV  detection is used for the determination of C3G (530 nm) levels in a lot of biological samples. This Methodology provides  accurate, reliable, and reproducible results of Cyanidin-3-O-glucoside (Chrysanthemin) measurement, which enables  us to analyze of C3G levels in vitro  and in vivo.

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