A domain is the basic building block for a protein structure, although many proteins only contain a single domain. Domains have different functional and structural units in a given protein. Usually they are act as the operational units responsible for a specific function or interaction, contributing to the overall role of a protein. Some of them are also linked to phenotypic traits and diseased states. Domains may exist in various biological contexts, where similar domains can be found in proteins with different functions. For instance, Src homology 3 (SH3) domains are relatively small domains with about 50 amino acid residues involved in protein-protein interactions. These domains have a typical 3D structure and occur in a diverse range of proteins with distinct functions, including phosphatidylinositol 3-kinases, adaptor proteins, phospholipases and myosins.
Enrichment analysis is a widely used and well established technique to test for the statistically significant over-representation of specific groups of genes or proteins, e.g. Pathways. Creative Proteomics can provide multiple enrichment analysis to assist our customers understanding protein functions and gaining the knowledge of biological pathways of interest. Now, bioinformaticians at Creative Proteomics are proud to offer our customers all above enrichment analysis.
The general steps of domain enrichment analysis provided by Creative Proteomics are summarized below:
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