Creative Proteomics offers an integrated solution to the identification of low abundance proteins in complex biological sample through 2D electrophoresis. This technique sorts proteins according to two independent properties in two discrete steps. Each spot on the resulting two-dimensional array corresponds to a single protein species in the sample. Thus thousands of different proteins can be separated, and the information is obtained including the protein pI, the apparent molecular weight, and the amount of each protein.
What Is 2D Electrophoresis?
Two-dimensional gel electrophoresis, abbreviated as 2-DE, is a form of gel electrophoresis commonly used to analyze mixtures of proteins by two properties in two dimensions respectively. In two-dimensional gel electrophoresis, proteins are first separated by their pI in isoelectric focusing and then further separated by molecular weight through SDS-PAGE, thus the sample proteins are distributed across the two-dimensional gel profile. This technique expands the number of proteins that can be identified, and provides more efficient data and detailed information for proteomics analysis.
2-D electrophoresis begins with 1-D electrophoresis and then separates the molecules by a second property in a direction 90 degrees from the first. In 1-D electrophoresis, the proteins separated in one dimension will lie along a lane, and then the molecules are spread out across in the 2-D gel. Generally, it is unlikely that two molecules will be similar in both two distinct properties, so molecules are more effectively separated in 2-D electrophoresis than in 1-D electrophoresis.
2D Electrophoresis Service at Creative Proteomics
2-DE is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. At Creative Proteomics, we can provide services 2D electrophoresis service according to standard operating procedures (SOPs).
Based on professional knowledge and experienced staff, Creative Proteomics provides high-resolution 2-DE for separating all kinds of proteins. Our ordering procedure is as follows. As every project has different requirements, please contact our specialists to discuss your specific needs. If you have any questions or specific needs, don’t hesitate to contact us.