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Home > Application > Analysis Services > Organic Acid Analysis Service > Isovaleric Acid Analysis Service

Isovaleric Acid Analysis Service

Isovaleric acid is also known as β-methylbutyric acid and 3-Methylbutanoic acid. It is one kind of organic acid and the formula is (CH3)2CHCH2CO2H. Isovaleric acid is classified as a fatty acid. It is highly soluble in most common organic solvents, but is sparingly soluble in water. Isovaleric acid is a colorless liquid under room temperature. Isovaleric acid can be found in essential oils and occurs naturally. Isovaleric acid has a sweaty smell or strong pungent cheesy, however, volatile esters, which is esters of isovaleric acid, have more pleasing scents compared isovaleric acid. Volatile esters are used in perfumery widely due to the pleasing scents. Isovaleric acid is a major reason to cause the unpleasant foot odor because it can be produced by the metabolized of leucine by skin bacteria.

Isovaleric acid Analysis Service

Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Isovaleric acid levels in various samples, including Tissue, Plant, Bacterial, Cells and more. High-Performance Liquid Chromatography (HPLC) using a differential refractive index detector (RID) for the determination of Isovaleric acid levels in a lot of biological samples.

Isovaleric acid is proposed as the first reason to produce the flavors of the wine. Brettanomyces yeasts cause the flavors. 4-ethylphenol, 4-vinylphenol, and 4-ethylguaiacol are other compounds produced by Brettanomyces yeasts when added to wine. The excess of isovaleric acid added in wine can be seen as a defect in general. The smell of isovaleric acid in wine is leathery, sweaty or like a barnyard when in a large amount. However, it can smell smoky, spicy, or medicinal when in small amounts added in wine. The killing of Brettanomyces yeasts can prevent the phenomena. The sterile filtration and the addition of relatively large quantities of sulfur dioxide can cause the death of Brettanomyces yeas. Isovaleric acid is also can be found in beer except some English–style ales, which is known as a flaw usually. Isovaleric acid can be formed though the oxidation of hops resins. The microbial oxidation of isovaleric acid results in the synthesis of β-hydroxyisovaleric acid, which is also known as β-hydroxy β-methylbutyric acid.

Sample Type
Tissue, Plant, Bacterial, Cells and more

Method
High-Performance Liquid Chromatography (HPLC) using a differential refractive index detector (RID) for the determination of Isovaleric acid levels in a lot of biological samples, Tissue, Plant, Bacterial, Cells and more. This Methodology provides accurate, reliable, and reproducible results of Isovaleric acid measurement, which enables us to analyze of Isovaleric acid levels in vitro and in vivo.

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