Isobutyric Acid Analysis Service

Q: Can you handle low-abundance samples?

Yes, our GC-MS platform achieves LOD < 0.1 µM even in 50 mg fecal samples.

Q: What bioinformatics support is included?

We provide pathway enrichment analysis, PCA, and correlation heatmaps.

Q: Can you handle complex matrices like fermentation broths or plant extracts?

Yes. Our protocols include solid-phase extraction (SPE) and derivatization to stabilize isobutyric acid in challenging matrices, achieving >95% recovery rates in plant tissues, fermentation fluids, and bacterial cultures

Creative Proteomics specializes in isobutyric acid analysis using GC-MS and LC-MS technologies. We provide precise metabolic profiling, biomarker identification, and pathway analysis to support pharmaceutical and biochemical research. Our services help researchers and industries accurately measure and interpret isobutyric acid levels, ensuring reliable data for metabolic studies and product development.

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What We Provide
Advantages
Technology Platform
Sample Requirements
FAQ
Publications

What is Isobutyric Acid?

Isobutyric acid, also known as 2-methylpropanoic acid, is a branched-chain short-chain fatty acid (BSCFA) with the molecular formula C₄H₈O₂. It is a product of the microbial fermentation of branched-chain amino acids like valine, leucine, and isoleucine in the gastrointestinal tract. While present in lower concentrations compared to other short-chain fatty acids, it plays a role in gut health and metabolic regulation.

Beyond its biological relevance, isobutyric acid is widely used as a precursor in the production of esters for flavoring, perfumes, and industrial applications such as disinfectants and plasticizers. Its unique chemical properties, including its water solubility and characteristic odor, make it valuable in both scientific research and commercial processes.

Isobutyric Acid Analysis Service Offered by Creative Proteomics

Isobutyric Acid Quantitative Analysis: Precise measurement of isobutyric acid levels in various samples using advanced GC-MS and LC-MS techniques.
Metabolic Profiling: Detailed analysis of isobutyric acid and related metabolites to explore metabolic pathways and microbial activity.
Biomarker Identification: Detection and quantification of isobutyric acid as a potential biomarker in clinical and metabolic research.
Pathway Analysis: Examination of isobutyric acid's role in metabolic and biochemical pathways, assisting in therapeutic and pharmaceutical studies.
Custom Solutions: Tailored analysis based on specific research needs, including method development and validation.
Data Interpretation and Reporting: Expert analysis with clear and actionable reports for informed decision-making.

List of Isobutyric Acid and Related Metabolites

List of Isobutyric Acid and Related Metabolites
Isobutyric Acid	Valine	Isobutyryl-CoA	Isobutanol	Isobutyraldehyde
Isobutyryl Carnitine	3-Hydroxyisobutyric Acid	3-Hydroxyisobutyryl-CoA	3-Amino-isobutyric Acid	Methylmalonyl-CoA
Succinyl-CoA	Propionyl-CoA	Acetyl-CoA	Isovaleric Acid	Butyric Acid
Propionic Acid	Methacrylic Acid	Isobutyrate Esters	α-Ketovaleric Acid	α-Ketoisovaleric Acid
β-Hydroxyisobutyric Acid	β-Hydroxyisovaleric Acid	Isovaleroyl-CoA	Tiglyl-CoA	Crotonyl-CoA
Glutaryl-CoA	Acetoacetyl-CoA	Malonyl-CoA

Advantages of Isobutyric Acid Assay

Ultra-Low Sample Consumption: Quantify isobutyric acid from ≥10 mg feces or ≥50 µL serum, ideal for rare/limited samples.
Dual-Platform Validation: Cross-confirmed results via GC-MS (Agilent 7890B/5977A) and LC-MS (Thermo Q Exactive) to eliminate platform-specific bias.
Broad Matrix Compatibility: Validated for feces, serum, tissue, plant extracts, and fermentation broths with <5% CV across matrices.
High Specificity: MRM-based detection avoids interference from structurally similar SCFAs (e.g., butyrate, isovalerate).
Custom Derivatization: Optimized protocols for volatile SCFA stabilization in complex biological fluids.
Scalable Throughput: Process 100–1,000+ samples per batch without compromising sensitivity (LOD: 0.1 μM).
Dynamic Range: Linear quantification from 0.1–1,000 μM, covering physiological to pathological concentrations.
Integrated Multi-Omics Support: Correlate SCFA data with proteomics or transcriptomics datasets.

Technology Platforms for Isobutyric Acid Analysis Service

HPLC-UV (High-Performance Liquid Chromatography with UV Detection)

Agilent 1260 Infinity II HPLC (Figure from Agilent)

GC-MS (Gas Chromatography-Mass Spectrometry)

7890B Gas Chromatograph + 5977 Single Quadrupole

Agilent 7890B-5977B (Figure from Agilent)

LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry)

SCIEX Triple Quad™ 6500+ (Figure from Sciex)

LC-MS

Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (Figure from Thermo)

Sample Requirements for Isobutyric Acid Analysis Service

Sample Type	Sample Volume	Storage Conditions	Required Sample Preparation
Blood Plasma/Serum	100 µL - 1 mL	-80°C for long-term storage	Centrifugation and separation required
Urine	1 mL	-20°C for short-term storage	No special preparation needed
Fecal Samples	200 mg	-80°C for long-term storage	Homogenization required
Cell Culture Media	1 mL	4°C for short-term storage	No special preparation needed
Tissue Samples	50 - 100 mg	-80°C for long-term storage	Tissue homogenization required
Saliva	1 mL	-20°C for short-term storage	No special preparation needed
Plasma/Serum (Mouse)	50 µL - 500 µL	-80°C for long-term storage	Centrifugation and separation required

Applications of Isobutyric Acid Assay Service

Metabolic Pathway Research

Study the role of isobutyric acid in metabolic pathways and its regulation in energy metabolism.

Biomarker Discovery

Identify and quantify isobutyric acid as a potential metabolic biomarker in animal and human studies.

Microbial Activity Analysis

Monitor the production of isobutyric acid by gut microbiota or other microorganisms in fermentation and digestion studies.

Food and Beverage Industry

Analyze the levels of isobutyric acid in fermentation processes for flavor and aroma production.

Environmental Research

Assess the impact of environmental factors on microbial production of isobutyric acid in soil or water samples.

Industrial Biotechnology

Evaluate isobutyric acid in the production of bio-based chemicals, including its use as a precursor in polymer manufacturing.

Demo

Overlay of chromatograms for fatty acids in a standard mix and serum sample, showing data acquired in PRM mode on a Thermo Hypersil aQ column, with some interference.

Overlay of extracted ion chromatograms for various fatty acids in a standard mix and pooled serum sample, acquired in PRM mode on a Thermo Hypersil aQ column (Shafaei, Armaghan, et al., 2021).

Overlay of chromatograms for fatty acids in a serum sample, showing acetic acid interference.

Overlay of extracted ion chromatograms for fatty acids in a pooled serum sample, separated on an ACE C18-AR column in PRM mode (Shafaei, Armaghan, et al., 2021).

FAQ of Isobutyric Acid Analysis Service

Can you handle low-abundance samples?

Yes, our GC-MS platform achieves LOD < 0.1 µM even in 50 mg fecal samples.

What bioinformatics support is included?

We provide pathway enrichment analysis, PCA, and correlation heatmaps.

Can you handle complex matrices like fermentation broths or plant extracts?

Yes. Our protocols include solid-phase extraction (SPE) and derivatization to stabilize isobutyric acid in challenging matrices, achieving >95% recovery rates in plant tissues, fermentation fluids, and bacterial cultures

How do you ensure specificity in distinguishing isobutyric acid from similar SCFAs?

We employ MRM (Multiple Reaction Monitoring) on GC-MS to target unique ion transitions of isobutyric acid, effectively avoiding cross-reactivity with butyrate or isovalerate. LC-MS further enhances specificity through high-resolution mass filtering

Learn about other Q&A.

Isobutyric Acid Analysis Service Case Study

Article

Quantitative Determination of Short-Chain Fatty Acids in Human Serum by LC-MS

Publications

Here are some of the metabolomics-related papers published by our clients:

A non-probiotic fermented soy product reduces total and ldl cholesterol: A randomized controlled crossover trial. 2021. https://doi.org/10.3390/nu13020535
Resting natural killer cell homeostasis relies on tryptophan/NAD+ metabolism and HIF‐1α. 2023. https://doi.org/10.15252/embr.202256156
Enhance trial: effects of NAD3® on hallmarks of aging and clinical endpoints of health in middle aged adults: a subset analysis focused on blood cell NAD+ concentrations and lipid metabolism. 2022. https://doi.org/10.3390/physiologia2010002
Dimethyl fumarate treatment restrains the antioxidative capacity of T cells to control autoimmunity. 2021. https://doi.org/10.1093/brain/awab307
Function and regulation of a steroidogenic CYP450 enzyme in the mitochondrion of Toxoplasma gondii. 2023. https://doi.org/10.1371/journal.ppat.1011566

More Publications

References

Shafaei, Armaghan, et al. "Sensitive and quantitative determination of short-chain fatty acids in human serum using liquid chromatography mass spectrometry." Analytical and Bioanalytical Chemistry 413 (2021): 6333-6342. https://doi.org/10.1007/s00216-021-03589-w
Dei Cas, Michele, et al. "A straightforward LC-MS/MS analysis to study serum profile of short and medium chain fatty acids." Journal of Chromatography B 1154 (2020): 121982. https://doi.org/10.1016/j.jchromb.2020.121982

Metabolomics Sample Submission Guidelines

Download our Metabolomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted metabolomics.

Download

Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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