Analysis of peptide amino acid sequences is the basis of peptide research. Peptide sequencing is used for a variety of purposes, including identifying new peptides, tracking post-translational modifications of peptides, and analyzing enzyme processes
Through the use of high-resolution mass spectrometry technology, Creative Proteomics is able to provide customers with high-fidelity, reproducible peptide sequencing services. Generally, peptide sequencing involves splitting a peptide chain chemically or enzymatically, and after that, determining the residue composition and content of amino acids. We provide a variety of peptide sequencing services.
What is Peptide Sequencing?
Peptide sequencing is the analysis of the sequence of amino acids that make up a peptide, which is also called the primary structure analysis of the peptide. The amino acid sequence of a peptide is the basis of its advanced structure and biological function, so peptide sequencing helps to further understand the advanced structure and biological function of the peptide.
How Do We Perform Peptide Sequencing?
Peptide sequencing involves determining the amino acid sequence of a peptide, which provides important insights into its structure, function, and interactions.
We will provide customers with sample preparation services, including extraction, digestion, purification and isolation of peptides, based on their needs and the nature of the samples provided by them.
Mass Spectrometry
Creative Proteomics has an advanced MS technology platform that uses different mass spectrometry methods, such as tandem mass spectrometry (MS/MS) to obtain the fragmentation pattern of peptides.
Peptide Fragment Analysis
Our experts analyze the fragment patterns obtained by MS to deduce the amino acid sequence of each peptide. This process, known as de novo sequencing, involves interpreting mass spectra to identify sequence-specific ion series and assigning amino acids accordingly.
Database Search
The obtained peptide sequences were searched in peptide databases using bioinformatics tools. This step helps identify the source peptide by comparing the experimental peptide sequence to the known peptide sequence.
Verify and Confirm
The final peptide sequence is verified through rigorous data evaluation, multiple experiments and independent verification methods to ensure the accuracy and reliability of the obtained peptide sequence.
Our Peptide Sequencing Services
We provide mass spectrometry-based peptide sequence analysis services and peptide de novo sequencing services, using the flourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) technology platform with high mass resolution and precision, making the experimental results highly reliable Reliability.
Our peptide N-terminal sequencing services are mainly classified into two categories, one is the classic Edman degradation method, the other is mass spectrometry, each has its advantages and limitations.
Peptide C-terminal sequencing was performed using tandem mass spectrometry. Our efficient and accurate C-terminal sequencing service is based on advanced ESI and MALDI TOF, which can detect blocked and modified peptide c-terminal, confirm whether the recombinant peptide is fully expressed, detect whether the recombinant peptide is broken during the expression process, and the c-terminal of the recombinant peptide whether the sequence is modified, etc.
Advantages of Our Peptide Sequencing Services
- Our peptide sequencing service can directly analyze the sequence of peptides without using any database information.
- Our service can be applied to analyze peptide sequences of new species and species whose genomes have not been sequenced, expanding the scope of research.
How to Order Our Services?
After receiving your peptide samples, our experts will discuss the optimal analysis strategy before performing characterization. We will keep you updated at each step and answer any queries you may have.
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Creative Proteomics has skilled experimental technicians who have accumulated rich experience in the field of peptide sequencing and can provide mass spectrometry-based peptide sequencing services. Combined with the bioinformatics database, the rapid and accurate analysis of the primary structure of the peptide can be realized. Please contact us and tell us your experimental needs!
* For research use only. Not for use in diagnostic procedures!
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