Creative Proteomics, your premier partner in cutting-edge proteomics analysis. Our commitment to innovation and excellence drives our fungi peptidomics analysis service, offering an unparalleled understanding of the peptidome in fungi. With state-of-the-art techniques and a team of experienced scientists, our fungi peptidomics analysis is designed to provide comprehensive insights into the peptidic landscape of various fungi species, fostering advancements in pharmaceuticals, agriculture, and biotechnology.
Plants Peptidomics Solutions
Fungi peptidomics analysis is a specialized branch of proteomics that focuses on the comprehensive characterization and quantification of endogenous peptides within fungi. These peptides play critical roles in various biological processes, including signaling, defense, and regulation. Understanding the peptidome of fungi is integral to unveiling novel bioactive compounds and elucidating complex biological mechanisms.
Key Focus:
- Yeast (Saccharomyces cerevisiae, Baker's yeast) Analysis
Our scientists can identify and quantify proteins and peptides in yeast through peptidomics analysis, and understand their expression changes during the fermentation process and their response to the external environment.
- Fungal Pathogens (e.g., Candida albicans, Cryptococcus) Analysis
Our scientists study the expression patterns of specific proteins in fungal pathogens through peptidomics analysis to gain insights into their role in infecting the host. By comparing peptide spectra before and after infection, proteins or peptides that may be associated with pathogenicity can be identified.
- Trichoderma Analysis
Our scientists use peptidomics to analyze the proteins and metabolites produced by Trichoderma during enzymatic hydrolysis, thereby optimizing the enzymatic hydrolysis reaction conditions and understanding the degradation mechanism of Trichoderma.
- Aspergillus Flavus (Autumn Mold, Aspergillus Flavus) Analysis
Aspergillus flavus is an aflatoxin-producing fungus that is present in many foods and feeds and poses a potential threat to human and animal health. Peptidomics analysis can be used to detect and identify the expression of toxin-related proteins or peptides in Aspergillus flavus strains, thereby monitoring and controlling aflatoxin content in food and feed.
Peptide Applications in Yeast Analysis
| Peptide Name |
Number of Amino Acids or Size |
Function |
| Signal Peptides |
10-40 |
Protein transport and localization |
| Inhibitory Peptides |
16–47 |
Functions and regulatory mechanisms study of proteins |
| Interaction Peptides |
45–54 |
Interacting peptides play an important role in yeast's two-hybrid system process and can help identify interacting regions and key amino acid residues of proteins. |
| Bioactive Peptides |
10-20 |
Some active peptides produced by yeast have specific biological activities such as antibacterial, antioxidant, and inhibition of tumor cell growth. |
| Yeast Peptides |
28–37 |
Studying yeast peptides can help understand the physiological processes, metabolic pathways and ability of yeast to cope with the external environment. |
Analysis Methods
- Liquid Chromatography-Mass Spectrometry (LC-MS/MS)
Utilizing high-resolution LC-MS/MS, we achieve sensitive and comprehensive detection of fungal peptides, enabling their accurate characterization and quantification.
- Tandem Mass Spectrometry (MS/MS)
Employing MS/MS fragmentation techniques, we elucidate the sequence and structural information of fungal peptides, facilitating their confident identification and structural annotation.
- Label-Free Quantification
Our label-free quantification methods provide accurate and reproducible quantitation of fungal peptides, allowing for comparative peptidome analysis across diverse samples.
Techniques and Instrumentation for Fungi Peptidomics Analysis
- Mass Spectrometry Systems
- Orbitrap Fusion Lumos Tribrid Mass Spectrometer: This system offers ultra-high resolution and exceptional sensitivity, enabling in-depth peptidome characterization and quantification.
- Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer: With its high-performance capabilities, this instrument facilitates precise identification and quantification of fungal peptides.
- TripleTOF 5600+ System: Equipped with high-speed mass spectrometry, this system enables accurate detection and analysis of fungal peptidome with exceptional depth.
- Liquid Chromatography Systems
- Agilent 1290 Infinity II LC System: Offering exceptional flexibility and sensitivity, this LC system is instrumental in achieving superior peptide separation and detection.
- Data Analysis Software
- Mascot: As a powerful search engine, Mascot aids in the confident identification and annotation of fungal peptides from mass spectrometry data.
- Skyline: This software offers advanced features for targeted quantification and validation of fungal peptide abundance, facilitating precise and reproducible data analysis.
At Creative Proteomics, we recognize the immense potential of plants peptidomics analysis in advancing the understanding of fungi. Our services are geared towards equipping researchers and organizations with the tools and expertise necessary to conduct in-depth peptidomics studies, thereby enabling groundbreaking discoveries in plant science. please contact us today and unlock the potential of peptide analysis.
* For research use only. Not for use in diagnostic procedures!
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