At Creative Proteomics, we provide high-quality peptidomics-based antigen discovery and prediction services to empower scholars in the realm of immunology and vaccine advancement. Our all-encompassing solutions harness the potential of peptidomics to discern, delineate, and anticipate prospective antigens for an array of purposes, encompassing vaccine, diagnostic assay formulation, and comprehension of immune responses.
What is Peptidomics-Based Antigen Discovery and Prediction?
Peptidomics-based antigen discovery and prediction involves the identification and prediction of antigens using peptidomics techniques. Peptidomics constitutes an area of inquiry that centers upon the identification and delineation of peptides, brief chains of amino acids, within a given biological specimen. In the context of antigen exploration, peptidomics methodologies are employed to scrutinize the range of peptides presented by cells, specifically those classified as antigen-presenting cells, such as dendritic cells. Peptidomics-based antigen discovery and prediction entail the employment of techniques to ascertain, define, and harness distinctive peptide sequences from antigen-presenting cells, thereby enhancing comprehension of the immune response and engendering novel strategies for vaccines and diagnostics.
Peptidomics-Based Antigen Discovery and Prediction by Creative Proteomics
- Peptide Extraction and Purification
We employ state-of-the-art techniques to extract and purify peptide samples from diverse biological sources, such as tissues, fluids, or cell lines. Our experts ensure high yield and purity of peptides for downstream analysis.
- Epitope Mapping
Through peptide synthesis and epitope mapping experiments, we precisely determine the binding sites of potential antigens with antibodies or major histocompatibility complex (MHC) proteins. This information aids in the design of highly specific and effective vaccines.
- Peptide-antigen Interaction Studies
We offer comprehensive studies to investigate the interaction between peptides and antigens. Through techniques such as ELISA, surface plasmon resonance (SPR), or molecular docking simulations, we can assess the binding affinity and specificity of the identified peptides towards specific antigens.
- Validation Studies
We provide validation studies to assess the antigenicity and immunogenicity of the predicted peptides. These studies can include in vitro assays using immune cells or in vivo animal models, depending on the specific requirements of the project.
- Epitope Prediction
Using computational algorithms and databases, we can predict potential epitopes within the identified peptides. This information is valuable for vaccine design and development, as well as for understanding the underlying immune responses.
Our Techniques in Peptidomics-Based Antigen Discovery and Prediction
- Peptide Extraction and Purification: We employ state-of-the-art techniques to extract and purify peptide samples from diverse biological sources, such as tissues, fluids, or cell lines. Our experts ensure high yield and purity of peptides for downstream analysis.
- MALDI-TOF/TOF: Thermo Scientific LTQ Orbitrap XL, Bruker Autoflex III.
- LC-MS/MS: Thermo Scientific Q Exactive Plus, Waters Xevo TQ-XS, Agilent 6495C Triple Quadrupole.
- High-resolution Mass Spectrometry: Thermo Scientific Orbitrap Fusion Lumos, Bruker Impact II.
- Bioinformatics and Machine Learning: By combining protein databases, sequence alignment algorithms, and machine learning approaches, we predict potential antigenic regions and epitopes.
- Sequence alignment tools
- Epitope prediction algorithms
- Peptide Synthesis and Epitope Mapping: Epitope mapping techniques such as peptide microarrays and alanine scanning allow for precise determination of binding sites.
- Peptide microarray synthesizers
- Alanine scanning mutagenesis kits
Applications of Peptidomics-Based Antigen Discovery and Prediction
- Vaccine Development: Peptidomics-based antigen discovery facilitates the design and development of highly effective vaccines by identifying antigenic regions and specific epitopes that trigger a desired immune response.
- Immunodiagnostics: Peptide antigens identified through peptidomics can be used as targets for diagnostic assays, enabling the development of highly specific and sensitive tests for various diseases, including infectious diseases and cancer.
- Immune Monitoring: Peptidomics helps in monitoring immune responses to diseases, vaccines, or therapeutic interventions by profiling the changes in peptide expression and identifying potential biomarkers.
- Autoimmune Disorders: Peptidomics analysis can aid in understanding the underlying mechanisms and identifying autoantigens associated with autoimmune disorders, enabling early detection and targeted therapies.
Why ChooseUs?
- We have scientific team with extensive experience in peptide and antigen
- Fully customizable designs to meet each specific need
- Advanced platform to ensure optimal results for every step
- Fast turnaround time and reasonable price
- Reliable and up-to-date analysis reports
- Friendly, expert advice is available
Our R&D department focuses on peptidomics-based antigen discovery and prediction. All development work is designed to meet the specific needs of Creative Proteomics' business and research partners. Our goal is to empower researchers, pharmaceutical companies, and vaccine developers in their efforts to discover novel antigens and advance immunological research. If necessary, please contact us.
* For research use only. Not for use in diagnostic procedures!
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