Label-Free Peptide Quantification Service

Welcome to Creative Proteomics, a leading provider of innovative proteomics solutions. As part of our comprehensive suite of services, we offer label-free peptide quantification service. Leveraging cutting-edge technologies and a team of experienced scientists, our label-free peptide quantification services provide accurate and reliable measurements without the need for specialized labels or dyes.

What is Label-Free Peptide Quantification?

Label-free quantification is a method in MS that determines the relative amounts of proteins in two or more biological samples, but unlike other quantification methods, it does not use stable isotopes that chemically bind and label proteins. Typically, peptide signals are detected at the MS1 level, whose isotopic patterns distinguish them from chemical noise. The pattern is then tracked in the retention time dimension and used to reconstruct the chromatographic elution profile of the monoisotopic peptide mass. The total ions of the peptide signal were then integrated and used as a quantitative measure of the original peptide concentration. For each detected peptide, all isotopic peaks are first found and then the charge state is assigned. In contrast to differential labeling, in label-free experiments each sample must be measured individually. The extracted peptide signal is then mapped to the LC-MS measurement using coordinates on the m/z ratio and retention time. Differential processing of biological samples demonstrates the need for a standard that can be used to adjust the results.

Label-Free Peptide Quantification Services by Creative Proteomics

• Peptide Separation
  We utilize advanced techniques in peptide separation.
  
  • Chromatographic separation
    We use high performance liquid chromatography (HPLC) technology, ion exchange chromatography (IEX), and reverse phase chromatography (RPC) to separate and enrich peptides in complex samples.
  • Size exclusion chromatography
    Size exclusion chromatography (SEC) is an analytical technique used for the separation between macromolecules and small molecules.
  • Affinity chromatography
    We provide a variety of affinity chromatography methods, such as metal chelation chromatography, antibody affinity chromatography, etc.
• Peptide Identification
  Our peptide identification services by mass spectrometry offer almost 100% success rate, by employing the latest technologies and optimized protocols. Samples are enzymatically digested, and run on a nanocapillary HPLC/MSMS, and the MSMS spectra are linked to a specific database of peptide identifications.
• Peptide Quantification
  We provide peptide quantification. Our team can precisely determine the quantity of your peptide. A variety of methods can be used to quantify peptides. For example, measuring at 280 nm in solution, weighing powdered peptides, or amino acid analysis methods when applicable.
• Mass Spectrometry Analysis
  We employ state-of-the-art mass spectrometry platforms, such as LC-MS/MS, to acquire spectra of labeled peptides for subsequent analysis. Our high-resolution mass spectrometers enable confident identification and accurate quantification of peptides.
• Statistical Analysis
  Statistical methods are applied to identify differentially abundant peptides between different samples or experimental conditions. This helps in understanding protein expression changes or biomarker discovery.

Techniques and Instrumentation for Label-Free Peptide Quantification Service

It's important to note that different instrument models and techniques may vary in their capabilities and performance, and the choice of instrument would depend on the specific research goals and requirements of the study.

• Mass Spectrometry (MS): It is used for label-free peptide quantification.
• SCIEX TripleTOF 5600
• Thermo Scientific Q Exactive
• Waters SYNAPT
• Data-Dependent Acquisition (DDA)
• Thermo Scientific LTQ Orbitrap XL
• SCIEX TripleTOF 6600
• Agilent 6550 Q-TOF
• Liquid Chromatography (LC) - Mass Spectrometry (LC-MS)
• Nano-Liquid Chromatography (nanoLC): Our instrument models include Thermo Scientific Dionex Ultimate 3000 RSLCnano, Waters nanoACQUITY UPLC System, Agilent 1200 Series nanoLC.
• Ultra-High Performance Liquid Chromatography (UHPLC): Our instrument models include Thermo Scientific Vanquish UHPLC System, Agilent 1290 Infinity II UHPLC System, SCIEX ExionLC, etc.
• Surface Plasmon Resonance (SPR)
  Biacore instruments by GE Healthcare (e.g., Biacore T200, Biacore 8K) are widely used for label-free peptide quantification using SPR technology.
• Differential Mobility Spectrometry (DMS)
  Agilent 6560 Ion Mobility Q-TOF LC/MS system is an example of an instrument model that utilizes DMS for label-free peptide quantification.
• Nanopore Sensing
  Oxford Nanopore Technologies instruments (e.g., MinION, GridION, PromethION) are used for label-free peptide quantification based on nanopore technology.

Why Choose Us?

• Peptide related impurities analysis
• Independent route design for complex target molecules and timely execution
• Solving problems with professional knowledge and creativity
• Complex biological data interpretation
• Responsive project management
• Rich experience in biosynthesis process and peptide related impurities analysis
• Broad therapeutic disease area of peptide expertise
• Experience in patent strategy

Applications of Label-Free Peptide Quantification Service

• Label-free peptide quantification testing has broad applications in biological and biomedical research, offering valuable insights into protein expression dynamics, disease mechanisms, and potential therapeutic targets.
• Quantification of protein expression levels: Label-free peptide quantification testing allows researchers to measure the absolute or relative abundance of specific proteins in biological samples.
• Comparative proteomics: Label-free peptide quantification testing can be used to compare protein expression levels between different samples, such as control and experimental groups. This allows researchers to identify proteins that are differentially expressed and potentially involved in specific biological processes or disease mechanisms.
• Biomarker discovery: Label-free peptide quantification testing can be used to screen and identify potential biomarkers for various diseases or conditions.
• Drug discovery and target identification: Label-free peptide quantification testing can be utilized in drug discovery research to evaluate the effects of drug candidates on protein expression levels.
• Systems biology and pathway analysis: Label-free peptide quantification testing can provide quantitative data on protein expression levels, which can be integrated with other omics data (such as genomics and metabolomics) to gain a systems-level understanding of biological processes. This can help in the identification and characterization of protein-protein interactions, signaling pathways, and regulatory networks.

Sample Requirements for Label-Free Peptide Quantification Assay

It is important to consult with our team regarding the detailed sample requirements, as they may have specific recommendations based on their assay protocols and instrumentation.

Quality Control

• Our team strictly adheres to standard operating procedures for sample handling and analysis.
• We have a trial process review system to ensure the smooth progress of the project.
• We verify analytical performance using reference standards and known samples.
• During the execution of the project, every link is monitored and tracked.
• Regular training and education of our laboratory staff to stay up-to-date with the latest techniques and best practices in label-free peptide quantification

* For research use only. Not for use in diagnostic procedures!

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