At Creative Proteomics, we understand the critical role that peptidomics plays in unraveling the intriguing world of plant biology. Our plant peptidomics analysis services are designed to provide comprehensive insights into the peptidome of various plant species, enabling researchers to unravel the complex regulatory networks and molecular mechanisms underlying plant growth, development, and responses to environmental stimuli.
Plants Peptidomics Solutions
Extracellular plant peptides have multiple functions, including signaling and defense. Intracellular peptides often have physiological functions or may simply be products of general proteolysis. Plant peptides have been identified and, in part, functionally characterized through biochemical and genetic studies that are lengthy and in some cases impractical. Peptidomics is a branch of proteomics developed in the past years and is mainly used to study the degradation of animal neuropeptides and proteases. Peptidomics is a rapid and efficient method that can detect minute and transient amounts of peptides and identify their post-translational modifications.
Key Focus:
- Oryza Sativa Peptidomics Analysis
Through peptidomics analysis, the protein composition in rice seeds can be identified, and the nutritional properties and growth characteristics of rice seeds can be understood. This can help improve rice varieties and enhance their nutritional content or disease resistance. Utilizing cutting-edge mass spectrometry platforms, we perform high-resolution analysis to identify and characterize plant peptides with unprecedented accuracy and sensitivity.
- Arabidopsis Thaliana Peptidomics Analysis
Peptidomics analysis of Arabidopsis thaliana can help study the expression of specific proteins during its growth and development, thereby understanding the plant's signaling pathways and response mechanisms to environmental stress. We employ advanced techniques for the extraction, isolation, and enrichment of plant-derived peptides, thereby enabling the comprehensive profiling of the plant peptidome.
- Solanum Lycopersicum Peptidomics Analysis
Through peptidomics analysis, the protein composition in tomato fruits can be revealed, as well as the differences in protein expression between different development stages or different varieties, providing information for improving tomato quality and yield. Our expert bioinformatics team utilizes sophisticated tools and databases to process and interpret the vast amount of peptidomics data, enabling precise annotation and functional analysis of plant-derived peptides.
- Hordeum Vulgare Peptidomics Analysis
The application of peptidomics analysis can help study the stress resistance of barley. For example, under drought or salt-alkali stress, peptidomics analysis can reveal changes in proteins in barley leaves or roots, thereby analyzing its stress resistance mechanism. We collaborate with our clients to tailor peptidomics analyses according to specific research goals, plant species, and experimental designs, ensuring a customized approach to meet unique requirements.
Plant Defence Peptide Applications in Plants
| Peptide Name |
Number of Amino Acids or Size |
Function |
| Hevein |
43 |
Antifungal |
| α- and β-thionin |
45–47 |
Pathogen defence, Seed storage protein |
| γ-Thionin (defensin) |
45–54 |
Antibacterial and fungal, Insecticidal activity |
| Lipid transfer Proteins (LTPs) |
LTP1 (9 kDa) |
Cuticle formation and embryogenesis |
| Cyclotides |
28–37 |
Insecticidal |
Analysis Methods
Our methodology for the analysis of plant peptidomics is grounded in a fusion of pioneering techniques crafted to unravel the intricate panorama of peptides derived from plants. These techniques encompass:
- Liquid Chromatography (LC)
Coupled with Tandem Mass Spectrometry (LC-MS/MS): LC-MS/MS serves as a flexible modality for discerning and quantifying plant peptides, furnishing exceptional sensitivity and precision for a comprehensive exploration of peptidomics.
- Capillary Electrophoresis (CE)-Mass Spectrometry
CE-MS boasts exceptional separation efficiency, rendering it an invaluable instrument for scrutinizing intricate plant peptidomes, particularly those containing scarce peptides.
- Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry
MALDI-MS permits swift and high-throughput examination of plant peptides, facilitating the profiling of diverse plant species with impressive expediency and precision.
Advantages of Plants Peptidomics Analysis
Plants peptidomics analysis confers a multitude of advantages, significantly enhancing our understanding of plant biology:
- Uncovering Bioactive Peptides: By identifying and characterizing bioactive peptides, plants peptidomics sheds light on the regulatory roles of these molecules in plant growth, development, and responses to environmental stimuli.
- Exploration of Signaling Pathways: Peptidomics analysis facilitates the elucidation of intricate signaling pathways and communication networks within plants, providing crucial insights into plant physiology and stress responses.
- Identification of Novel Biomolecules: Through peptidomics analysis, researchers can unearth novel peptides with potential applications in agriculture, biotechnology, and pharmaceutical research, thereby expanding the repertoire of bioactive compounds derived from plants.
At Creative Proteomics, we recognize the immense potential of plants peptidomics analysis in advancing the understanding of plant biology. Our services are geared towards equipping researchers and organizations with the tools and expertise necessary to conduct in-depth peptidomics studies, thereby enabling groundbreaking discoveries in plant science. please contact us today and unlock the potential of peptide analysis.
* For research use only. Not for use in diagnostic procedures!
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