Determining the accurate mass of peptides is essential for many applications in biopharmaceutical research and development. At Creative Proteomics, we offer highly accurate mass determination services for peptides using state-of-the-art mass spectrometry platforms. With decades of experience providing cutting-edge peptidomics solutions, our expert scientists utilize advanced techniques to deliver reliable characterization of peptide molecular weight.
How We Detect Peptide Molecular Mass
Creative Proteomics offers high-quality peptide sample preparation services. You only need to specify your experimental purpose and deliver the experimental samples to us, and we will handle the subsequent experiments. If you want to learn more details, please click peptide sample preparation services.
Molecular Weight Detection
Based on the advanced MS technology platform, we guarantee to provide customers with reliable and efficient peptide molecular weight detection services. We'll keep you updated every step of the way and answer any questions you may have. Data we can test on:
- Number average molecular weight (Mn)
- Weight average molecular weight (Mw)
- Z-average molecular weight (Mz)
- Polydispersity (Mw/Mn) on both single and multi-component polymer samples
- Accurate molecular mass determination service (peptides with modifications, such as glycosylation, phosphorylation)
- Molecular mass distribution service
Data Analysis and Report
After completing the mass analysis, a detailed report containing the following information will be provided:
- Sample preparation methods
- Mass spectrometry parameters and conditions
- MS chromatograms
- Tables with measured mass, deviation from expected mass and identification details
- Expert interpretation and conclusions
Peptide Sample Requirements for Molecular Weight Detection
Sample Preparation
If you choose to prepare the sample yourself, please avoid denaturation, irreducible damage, or adsorption of the peptide to the container wall during the sample preparation process. For example, the use of strong buffers or surfactants is not recommended, and samples should be stored in protein-stable containers such as glass or plastic. Please note that the samples provided will not be returned, so please make a backup copy.
Peptide Concentration
The concentration of peptides in the sample should be appropriate, usually between 0.1-1.0 mg/mL. A concentration that is too high may cause intermolecular aggregation or precipitation, while a concentration that is too low may cause the signal to be too weak to be measured accurately.
Peptide Purity
Samples must be of high purity to reduce interference from other components in molecular weight determination. You can also choose to have us purify and enrich your samples for you.
Sample Stability
The sample should have good stability, and reactions such as denaturation, degradation or aggregation should not occur during the measurement process, otherwise it will affect the molecular weight measurement results.
Peptide Molecular Weight Range
The molecular weight of the protein in the sample should be within the applicable range of the chosen assay method. Common protein molecular weight determination methods such as SDS-PAGE and mass spectrometry have different measurable molecular weight ranges. The common molecular weight range of peptides is 0.1-10 kD.
Sample Transportation and Storage Conditions
If possible, it is best to store and transport protein samples at low temperatures to reduce the possibility of denaturation. At the same time, in order to avoid microbial contamination and changes in chemical substances, multiple freezing and thawing should be avoided as much as possible.
Our MS Technology Platforms
Advantages of Our Peptide Molecular Mass Determination Service
- Low sample consumption.
- One-stop solution: sample processing, computer analysis, data analysis, project reporting.
- High-throughput technology.
Our cutting-edge platforms, experienced scientists and tailored solutions enable deep, accurate characterization of peptide molecular weight and composition. With Creative Proteomics as your peptidomics partner, you can rely on our accurate mass determination services for critical answers about your peptide molecules. If you have questions or special requirements about our peptide molecular mass determination service, please contact us!
* For research use only. Not for use in diagnostic procedures!
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