Peptide Absolute Quantification Service

Absolute quantification peptides serve as internal controls or peptide standards (quantitative reference peptides) for protein quantification in mass spectrometry-based proteomics, such as selected reaction monitoring (SRM), multiple reaction monitoring (MRM), and Parallel Reaction monitoring (PRM). The absolute quantitation of peptides is a potent approach for elucidating the precise quantity of peptides within a sample. Distinct from relative quantification techniques that offer insights into the abundance ratio of peptides, absolute quantification enables a more meticulous evaluation of peptide concentration. Our exclusive method for absolute peptide quantification efficiently surpasses the constraints of conventional methodologies.

What is Peptide Absolute Quantification?

Absolute peptide quantification is a method utilized in the field of proteomics to ascertain the precise quantity of a specific peptide that exists within a biological specimen. This technique entails the measurement and quantification of the targeted peptide through diverse methodologies such as mass spectrometry. During the process of peptide absolute quantification, a predetermined quantity of internally consistent stable isotope-labeled standard peptides is introduced to the sample. These internal standards possess an identical sequence of amino acids as the targeted peptide, yet they diverge in their isotopic composition, thus permitting their distinction during the analysis conducted by mass spectrometry. The internal standards serve as compounds of reference, and their established concentration serves as the basis for determining the absolute quantity of the desired peptide.

Peptide Absolute Quantification Services by Creative Proteomics

• Stable Isotope-Labeled Standard (SIS) Peptide Synthesis
  To enable absolute quantification, we can synthesize stable isotope-labeled peptides that serve as internal standards. These labeled peptides are chemically identical to the target peptides but differ in their isotopic composition, allowing for accurate quantification.
• Mass Spectrometry Analysis
  We utilize state-of-the-art mass spectrometry instruments, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS), to quantify the absolute abundance of peptides in your samples. Our highly sensitive and accurate instruments enable precise measurements even in complex biological matrices.
• Absolute Quantification Method Selection
  The appropriate quantification method is selected based on the specific requirements of the study. Common methods used for peptide absolute quantification include targeted mass spectrometry (e.g., selected reaction monitoring), stable isotope dilution, and label-free quantification.
• Standard Curve Preparation
  A series of known peptide standards with known concentrations is prepared. These standards will be used to generate a calibration curve, which relates the signal intensity of the peptides to their actual concentrations.

Advantages of Peptide Absolute Quantification Service

• Accurate Quantification: We offer precise and dependable quantitative information regarding the absolute abundance of target peptides in the sample.
• High Sensitivity: At Creative Proteomics, the utilization of sophisticated mass spectrometry methodologies enables the detection and measurement of peptides even at dilute concentrations.
• Custom Service: Our service can be customized to align with the distinctive requirements of the client, encompassing the selection of the quantification approach and target peptides.

Analysis Methods of Peptide Absolute Quantification

Creative Proteomics employs various advanced analysis methods for Peptide Absolute Quantification Service:

• Multiple Reaction Monitoring (MRM)
  This focused mass spectrometry approach concentrates on precise ion transitions of specific peptides, enabling meticulous quantification. Through the comparison of peak areas between the target peptides and internal standards, we can ascertain the unequivocal abundance of peptides in your samples.
• Parallel Reaction Monitoring (PRM)
  Parallel reaction monitoring (PRM), is similar to selected reaction Monitoring (SRM), and employs a focused approach in mass spectrometry, allowing for the simultaneous detection of numerous peptide ions. This technique presents exceptional selectivity, sensitivity, and multiplexing capacity, enabling precise and absolute quantification of peptides.
• Isotope Dilution Mass Spectrometry (IDMS)
  In IDMS, stable isotope-labeled peptides that are chemically identical to the target peptides are added to the sample as internal standards. By comparing the ratios of the labeled and unlabeled peptides, we can accurately determine the absolute abundance of the target peptides.

Applications of Peptide Absolute Quantification Services

Peptide absolute quantification encompasses the meticulous determination and quantification of the precise concentration of particular peptides within a biological specimen. This technique holds manifold applications across various domains, including:

• Biomarker Elucidation and Verification: Peptide absolute quantification aids in the identification and validation of potential biomarkers for diverse diseases and conditions.
• Drug Development and Pharmacokinetics Exploration: The absolute quantification of peptides facilitates the assessment of a drug's pharmacokinetics, encompassing its absorption, distribution, metabolism, and excretion within the body.
• Proteomics Advancements and Systems Biology Inquiries: Through peptide absolute quantification, researchers can glean profound insights into cellular processes, protein interactions, and regulatory networks, thereby augmenting our comprehension of biological systems.
• Disease Monitoring and Personalized Medicine Endeavors: Peptide absolute quantification becomes integral in monitoring the progression and treatment response of a diverse array of maladies, including cancer.
• Quality Control Within Biopharmaceutical Production: By quantifying the concentration of specific peptides within recombinant proteins or antibodies, researchers can ensure that the desired product is crafted at the prescribed concentration, thereby guaranteeing its safety and efficacy.

Our Capabilities in Comparison of Absolute Quantified Peptides

* For research use only. Not for use in diagnostic procedures!

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