Providing high-quality peptide sequencing solutions to address complex biological challenges, Creative Proteomics leverages advanced mass spectrometry （MS） technology and extensive team experience to offer accurate, efficient, and comprehensive services ranging from sample preparation to data analysis and result interpretation.
What is Peptide N-terminal Sequencing?
The amino acid sequence at a peptide's N-terminus can be determined through methods like Edman degradation, MS fragmentation, or enzymatic cleavage paired with sequencing - together known as peptide N-terminal sequencing techniques.
How Do We Perform Peptide N-terminal Sequencing?
Edman Sequencing Technology
As is described in the diagram below, the N-terminal amino acid residue is repeatedly cleaved, the sequence of a peptide is determined by identifying transferred PTH-amino acid (PTH-AA) residues.
- Mature technology, simple and convenient operation.
- Wide range of applications.
MS Technology
- Sample Preparation: We provide sample preparation services involving purification, concentration and removal of contaminants or interfering substances.
- Peptide fragmentation: The peptide sample is then fragmented using techniques such as CID or ETD.
- Mass spectrometry: A fragmented peptide sample is introduced into a mass spectrometer, which ionizes the peptides and separates them based on their mass-to-charge ratio (m/z).
- Data Acquisition: The MS collects mass and intensity data of the peptide fragments produced during the cleavage process.
- Verification and validation: To ensure the accuracy and reliability of the identified N-terminal sequences, validation steps such as manual inspection of spectra, comparison with theoretical fragmentation patterns, and consideration of post-translational modifications (PTMs) were performed by us.
Comparison of MS and Edman Degradation
Our MS technology offers high sensitivity, versatility, and high-throughput capabilities in protein N-terminal sequencing, but it requires specialized instrumentation and data interpretation. Edman degradation, on the other hand, provides sequential analysis and chemical specificity for the N-terminal residues but has limitations in sample length, time consumption, and sensitivity to certain modifications. The choice between these methods depends on factors such as sample characteristics, research goals, and available resources.
Sample Requirements
We accept peptide freeze-dried powder, peptide solution, or peptide immobilized on PVDF membrane.
- The sample needs to have a peptide amount of 1-10 μg, the sequencing sample will not be returned, please keep a backup.
- The purity is supposed to be more than 95%.
- Do not use surfactants in the buffer, and reduce the salt concentration of the solution as much as possible.
- If the apparent molecular weight of the peptide or the theoretical peptide sequence is known, please explain to improve the success rate of sequencing.
What Can Peptide N-terminal Sequencing be Used for?
- Reveal the high-level structure and biological functions of peptides
- Study PTMs of peptides
- Study isomers and variants of peptides
- Complementary drug development and biopharmaceuticals
- Peptide drug quality control
Why Choose Us?
- Suitable for large-scale peptidomics studies
- Combining bioinformatics to improve analysis efficiency
- Greater accuracy and sensitivity
Creative Proteomics provides the highest quality peptide N-terminal sequencing services, and has an experienced technical team and advanced equipment to meet your various needs. If you need to know more about peptide N-terminal sequencing services, please feel free to contact us! We will arrange a professional technical service team to provide you with detailed quotations and plans to ensure that your needs are met to the greatest extent.
* For research use only. Not for use in diagnostic procedures!
Our customer service representatives are available 24 hours a day, 7 days a
week.
