Creative Proteomics offers a comprehensive and cutting-edge multiple reaction monitoring (MRM)-based peptide quantification service. With a team of highly skilled experts and state-of-the-art equipment, we provide accurate and reliable quantification of peptides for various research and analysis purposes. Our MRM-based peptide quantification service combines advanced mass spectrometry techniques with robust data analysis to deliver precise and reproducible results.
What is MRM-Based Peptide Quantification?
Multiple reaction monitoring-based peptide quantification is an extensively employed technique in proteomics for the precise and directed assessment of particular peptides within intricate biological samples. This method entails the deliberate selection and vigilant monitoring of specific peptide precursor ions and their corresponding fragment ions (transitions) using a mass spectrometer. Multiple reaction monitoring-based peptide quantification grants remarkable precision and sensitivity, rendering it especially advantageous for the detection of potential biomarkers, quantification of proteins, and exploration of diverse cellular mechanisms. It is frequently applied in fields including clinical investigation, pharmaceutical exploration, and targeted proteomics.
MRM-Based Peptide Quantification Services by Creative Proteomics
- Peptide Quantification
Accurate measurement of peptide concentrations in complex biological samples using MRM-based methods.
- Absolute Quantification
Precise quantification of target peptides based on the use of internal standards and appropriate calibration curves.
- Relative Quantification
Comparative analysis of peptide abundance between different samples or experimental conditions.
- Targeted Proteomics
Profiling and quantification of specific proteins or peptides of interest in a high-throughput manner.
- Validation and Quality Control
We adhere to rigorous validation protocols to ensure the accuracy, precision, and reliability of our MRM assays. Our quality control procedures guarantee the delivery of high-quality results to our customers.
- Consulting and Training
We offer consulting services to assist our customers in experimental design, method optimization, and troubleshooting. Furthermore, we provide training programs to educate researchers and lab technicians on MRM-based quantification techniques.
- Customized Projects
We also undertake customized projects, collaborating closely with customers to design and execute MRM-based quantification studies tailored to their specific research aims.
Our Techniques for Multiple Reaction Monitoring-based Peptide Quantification Service
Our MRM-based peptide quantification service utilizes a wide range of techniques, including:
- Multiple Reaction Monitoring (MRM)
Targeted mass spectrometry technique for the specific detection and quantification of peptides.
- Stable Isotope-labeled Peptides
Internal standard peptides with stable isotopes incorporated, used for accurate quantification.
- Deuterium (D) labeling: Deuterium isotopes replace hydrogen atoms. Commonly used labeling agents are D2O and deuterium hydrogen dioxide.
- C-13 labeling: The carbon-13 isotope replaces the carbon atom.
- N-15 labeling: The nitrogen-15 isotope replaces the nitrogen atom.
- Selected Reaction Monitoring (SRM)
Similar to MRM, SRM allows for the specific detection and quantification of target peptides in complex samples.
- High-performance Liquid Chromatography (HPLC)
Separation technique that enhances the sensitivity and resolution of peptide detection.
- Agilent 1290 Infinity II LC System
- Shimadzu Prominence HPLC System
- Waters Acquity UPLC System
- Triple Quadrupole Mass Spectrometry (TQ-MS)
A mass spectrometry system consisting of three quadrupole mass analyzers, enabling precise and selective peptide quantification.
- Data Analysis Tools
Process of Multiple Reaction Monitoring-based Peptide Quantification Service
Our MRM-based peptide quantification service follows a well-defined process to ensure accurate and reliable results:
- Consultation and sample submission: We engage in a thorough and extensive dialogue concerning your research needs and requirements, guiding you through the intricate process of submitting your samples.
- Sample preparation: Our esteemed experts employ meticulously optimized protocols for sample preparation, deftly extracting and digesting peptides from your samples, thus ensuring the epitome of recovery and stability.
- Liquid chromatography (LC) Separation: Utilizing state-of-the-art LC systems, we adeptly separate peptides, skillfully resolving intricate mixtures.
- Mass spectrometry analysis: Employing triple quadrupole mass spectrometry, we quantitatively analyze target peptides using MRM-based methods, bolstered by stable isotope-labeled internal standards to ensure unparalleled accuracy in quantification.
- Data analysis and interpretation: Harnessing our sophisticated suite of data analysis tools, we facilitate precise quantification, meticulous statistical analysis, and expert interpretation of results.
- Report generation: Tailored to your specific requirements, we diligently generate comprehensive reports encompassing all pertinent data, results, and analyses.
Applications of Multiple Reaction Monitoring-based Peptide Quantification
- Biomarker exploration: MRM-based peptide quantification has the potential to unveil and assess particular peptides as biomarkers for various ailments.
- Pharmacokinetics and drug metabolism: MRM-based peptide quantification can be employed to ascertain the concentration of drug metabolites or therapeutic proteins in biological samples, aiding in comprehending the pharmacokinetics and metabolism of pharmaceuticals.
- Clinical diagnostics: MRM-based peptide quantification can be utilized in clinical settings to measure specific peptides relevant to disease diagnosis or monitoring.
- Protein verification/validation: MRM-based peptide quantification can be applied to authenticate the presence and quantify particular proteins of interest.
- Food quality and safety evaluation: It can be employed to quantitate allergenic proteins and microbial contaminants or identify specific constituents of food.
We understand the critical role precise and accurate quantification plays in the field of proteomics and offer comprehensive solutions to meet your research needs. Our experienced team of scientists, state-of-the-art facilities, and cutting-edge technologies enable us to deliver high-quality results with quick turnaround times. Whether you are conducting basic research or developing innovative therapeutics, our MRM-based peptide quantification service can provide valuable insights into protein expression and characterization. If necessary, please feel free to contact us.
* For research use only. Not for use in diagnostic procedures!
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