Immune Peptide Library Screening

Creative Proteomics is a leading provider of immune peptide library screening services. Our state-of-the-art facility is equipped with advanced technologies and a team of experienced scientists dedicated to accelerating drug discovery and development in the field of immunotherapy. Through robust screening processes and comprehensive analysis methods, we help researchers and pharmaceutical companies identify unique peptides that can modulate immune responses and serve as potential targets for therapeutic intervention.

What is Immune Peptide Library Screening?

Immuno-peptidome library screening is a sophisticated method employed to discern peptides exhibiting precise and selective binding to a desired target, be it a protein or a cellular receptor, from an expansive repertoire of diverse peptide sequences. This comprehensive library, composed of synthetically or genetically engineered peptides, encompasses a broad spectrum of sequences. The meticulous screening process entails subjecting the peptide library to the target molecule and subsequently isolating those peptides that manifest highly specific and strong binding affinities to the target. This selection can be achieved employing various cutting-edge methodologies, such as phage display, yeast display, or bacterial display. Following the identification of peptides with a robust affinity for the target, further characterization can be pursued, thus unleashing their potential in a multitude of applications.

Immune Peptide Library Screening Services by Creative Proteomics

• Library Design and Construction
• Customized design and construction of immune peptide libraries tailored to specific research goals.
• Incorporation of diverse peptide sequences to maximize coverage of the peptide space.
• Strategic selection of peptide lengths and modifications to enhance library performance.
• High-Throughput Screening
• Utilization of high-throughput techniques for efficient screening of immune peptide libraries.
• Assessment of peptide-antigen interactions, peptide-protein interactions, and peptide-receptor interactions.
• Generation of quantitative binding profiles and determination of peptide affinities.
• Functional Peptide Characterization
• Evaluation of peptide-induced immune responses, including cytokine production, cell activation, and proliferation assays.
• Determination of peptide-mediated immune modulation and immunomodulatory activities.
• Identification of peptides with potential therapeutic applications.
• Peptide Synthesis
  We use chemical and biological methods to synthesize large-scale peptide libraries. Our advanced peptide synthesis technology ensures high purity and stable peptides to meet customer needs.
• Peptide-Protein Binding Assays
• Employing various binding assays such as surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), and fluorescence polarization (FP).
• Quantification of peptide-protein interactions and determination of binding affinities.
• Characterization of binding kinetics and stability.

Analysis Methods in Immune Peptide Library Screening

• Next-Generation Sequencing (NGS)
• Application of NGS technologies to analyze enriched peptide sequences following library screenings.
• Identification of peptide hits and their frequencies, enabling the selection of high-affinity peptides for downstream studies.
• Comprehensive analysis of complex peptide populations to uncover novel targets and therapeutic leads.
• Cell-based Functional Assay
• At Creative Proteomics, our scientists evaluate the functional activity of peptides using immune cell lines or primary cells.
• We offer services for measuring cytokine release, cell proliferation and cell activation in response to peptide stimulation.
• We can screen for immunomodulatory properties and identify peptides with the desired immune response.

Process of Immune Peptide Library Screening

• Library Design and Construction
  Collaborative discussion between clients and our team to define research goals and select appropriate peptide sequences.
• Screening Assays
  High-throughput screening of the immune peptide library against the target of interest using various binding assays or functional assays.
• Data Analysis
  Identification of peptide hits based on binding affinity, functional activity, or immunomodulatory properties.
• Validation and Optimization
  Selected peptide hits were validated through external experiments to confirm their activity and specificity.

Our Immune Peptide Library Screening Platform

• Surface Plasmon Resonance (SPR)
  Instruments: Biacore S200, Biacore X100, Octet RED96
• Isothermal Titration Calorimetry (ITC)
  Instruments: MicroCal PEAQ-ITC, MicroCal ITC200, Nano ITC
• Fluorescence Polarization (FP)
  Instruments: Fluoroskan FL, Polarstar Omega
• Next-Generation Sequencing (NGS)
  Instruments: Illumina MiSeq, Ion PGM

Our Advantages

• Scientific team with extensive experience in handling peptides
• Fully customizable designs to meet each specific need
• Advanced platform to ensure optimal results for every step
• Fast turnaround time and reasonable price
• Reliable and up-to-date analysis reports
• Friendly, expert advice available

* For research use only. Not for use in diagnostic procedures!

Our customer service representatives are available 24 hours a day, 7 days a week.