In-depth understanding of the structural information of peptides plays an important role in exploring their potential applications. At Creative Proteomics, we provide cutting-edge peptide C-terminal sequencing services to help researchers, drug developers, and biotechnology companies perform high-precision analysis of the C-terminal amino acid sequence in polypeptide chains, providing customers with more accurate and comprehensive sequencing data.
What is Peptide C-terminal Sequencing?
C-terminal sequencing of peptides refers to the process of determining the amino acid sequence of the C-terminal of a peptide chain. With this technique, we can obtain important information about the composition, modifications, and potential functional domains of the peptide.
How Do We Perform Peptide C-terminal Sequencing?
Enzyme cleavage
We use specific proteases or peptidases to selectively cleave peptides at specific amino acid residues. Techniques such as trypsin digestion and carboxypeptidase cleavage are commonly used and can provide valuable information about the C-terminal residues.
Mass Spectrometry (MS) Technology
Mass spectrometry-based fragmentation techniques, such as tandem mass spectrometry (MS/MS), analyze fragment ions to infer the sequence of the peptide, thereby helping to determine the C-terminal sequence. MS technology offers excellent sensitivity, high-throughput capabilities, and compatibility with a wide range of peptide samples.
Process of Our Peptide C-terminal Sequencing Service
Sample Preparation
Collect and purify peptide samples that require sequencing, and perform appropriate processing according to customer needs.
Sample Processing
The peptide sample is mixed with a specific enzyme to break it at a specific site, exposing the C-terminal amino acid residue.
LC-MS Analysis
The processed samples were separated and detected by liquid chromatography coupled with mass spectrometry technology to obtain the accurate mass of each C-terminal amino acid fragment.
Data Analysis
Process and analyze the obtained LC-MS data, compare the generated peptide segments using the database, and finally determine the C-terminal amino acid sequence of the polypeptide.
For Peptides of Known Sequence
The theoretical molecular weight of the C-terminal peptide was calculated according to the sequence and restriction site, and then the peptide with the corresponding molecular weight was selected in the mass spectrometry analysis, and the correctness of the C-terminal was confirmed by the secondary mass spectrometry sequence analysis.
For Peptides of Unknown Sequence
A series of methods are used to generate characteristic peaks of C-terminal peptides, such as the carboxypeptidase method, and the use of isotopes to label the C-terminal of polypeptides. The precursor ions of the C-terminal peptide were subjected to sequence analysis by MS/MS.
What Can You Get?
After completing the mass analysis, a detailed report containing the following information will be provided:
- Sample preparation methods
- Mass spectrometry parameters and conditions
- MS chromatograms
- Expert interpretation and conclusions
Why Choose Us?
- The cycle is short, the precision is high, and the result is accurate.
- Suitable for all kinds of C-terminal sequence detection.
- With higher sensitivity and reproducibility, it can be applied at the peptidomics level.
Creative Proteomics provides important technical support for biotechnology research with its advantages of precision, efficiency and comprehensiveness. Whether it's basic research or drug development, we can provide you with reliable solutions. In order to better meet customer needs, we will continue to optimize our service processes and technology platforms to provide customers with a better service experience. If you are interested in our peptide C-terminal sequencing services or have questions or suggestions, please feel free to contact us!
* For research use only. Not for use in diagnostic procedures!
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