At Creative Proteomics, we provide cutting-edge services in the realm of neuropeptidomics. Our integrated analysis of neuropeptidomics service has been meticulously crafted to furnish a holistic comprehension of the neuropeptide composition found within biological samples, thus facilitating a profound insight into the intricate workings of the nervous system. Equipped with state-of-the-art methodologies and sophisticated instrumentation, our advanced techniques guarantee precise and dependable outcomes, thereby empowering researchers to unravel the enigmatic mechanisms that underlie the field of neurobiology.
What is Integrated Analysis of Neuropeptidomics?
Integrative services of neuropeptidomics encompass the all-encompassing investigation and explication of neuropeptides within a biological milieu. This approach entails the identification, quantification, and characterization of neuropeptides, as well as the exploration of their biological attributes and interconnections. By synergistically incorporating diverse analytical methodologies and advanced data analysis techniques, our experts are conferred the ability to comprehend the intricate neuropeptide networks and their requisite functions in physiological and pathological manifestations.
Integrated Analysis of Neuropeptidomics by Creative Proteomics
- Neuropeptide Identification
We employ advanced mass spectrometry technologies to identify neuropeptides present in your samples. By comparing the detected molecular ions and fragmentation patterns against comprehensive databases, we accurately determine the identity of neuropeptides.
- Neuropeptide Quantification
Our quantitative proteomics methods enable precise measurement of neuropeptide concentrations. Through label-free or isotopic labeling techniques, we provide quantitative data on neuropeptide expression levels, facilitating comparative analysis and understanding of dynamic changes.
- Targeted mass spectrometry quantitation
- Untargeted mass spectrometry quantitation
- Ion mobility spectrometry
- Single-cell mass spectrometry
- Neuropeptide Profiling
We offer comprehensive neuropeptide profiling services to gain a holistic view of the neuropeptide landscape in your samples. By employing data-dependent acquisition (DDA) or data-independent acquisition (DIA) methods, we can comprehensively analyze and quantify neuropeptides present in a sample.
Sample Requirements
The samples required for the integrated analysis of neuropeptidomics should be collected and handled with utmost care to ensure accurate and reliable results. The following are the recommended sample requirements:
| Content |
Requirements |
| Sample Type |
The samples can include various biological matrices such as tissues, biofluids (e.g., blood, cerebrospinal fluid), or cell cultures. |
| Sample Storage |
It is recommended to store samples at ultra-low temperatures (-80°C) or in liquid nitrogen for long-term storage. |
| Volume |
- Liquid Chromatography Mass Spectrometry (LC-MS): 1-10 uL
- MALDI-TOF mass spectrometry: 1-5 nL
- Capillary electrophoresis mass spectrometry (CE-MS): 10-100 nL
|
| Quality |
The sample should be of good quality, free from contaminants, and should not have undergone extensive degradation. |
| Sample Matrix |
Any interfering substances should be removed or minimized before analysis through sample preparation techniques such as protein precipitation, desalting, or purification |
Results Delivery
We understand the importance of timely delivery of results. After completion of the integrated analysis of neuropeptidomics service, we provide a comprehensive report, including raw data files, annotated spectra, neuropeptide identification, quantification, and statistical analysis, as applicable. Our team of experts is available to discuss and clarify the results to ensure a clear understanding of the findings.
- Sample preparation methods
- Mass spectrometry parameters and conditions
- MS chromatograms
- Tables with measured mass, deviation from expected mass and identification details
- Expert interpretation and conclusions
Techniques and Instrumentation for Integrated Analysis of Neuropeptidomics
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry (MALDI-MS)
- Shimadzu AXIMA series
- Bruker Ultraflextreme MALDI-TOF/TOF Mass Spectrometer
- Electrospray Ionization-Mass Spectrometry (ESI-MS)
- Agilent 6545 Quadrupole-Time-of-Flight (Q-TOF) Mass Spectrometer
- Tandem Mass Spectrometry (MS/MS)
- Employing collision-induced dissociation (CID)
- Higher-energy collision dissociation (HCD)
Our Neuropeptides Types
We have analyzed neuropeptides with multiple functions, as shown in the table below:
| ACTH |
CCK-8 |
Galanin |
Neurotensin |
| AGRP |
CGRP |
Neuropeptide S |
Orexin |
| CART |
CRF |
Neuropeptide Y |
Oxytocin |
| Somatostatin |
Substance P |
Vasopressin |
VIP |
Our R&D department focuses on optimizing all aspects of neuropeptidomics analysis. All development work is designed to meet the specific needs of Creative Proteomics' business and research partners. If necessary, please feel free to contact us.
* For research use only. Not for use in diagnostic procedures!
Our customer service representatives are available 24 hours a day, 7 days a week.
