NMR-Based Peptide Structure Analysis

Creative Proteomics' peptide structure analysis services are tailored to meet the needs of pharmaceutical companies, academic researchers and biotechnology companies seeking accurate and reliable peptide characterization. Drawing on our expertise and state-of-the-art nuclear magnetic resonance (NMR) instrumentation, we provide comprehensive peptide structure analysis, including sequence determination, conformational analysis and identification of secondary structure elements. Our team of highly skilled scientists employ cutting-edge techniques and data interpretation methods to provide detailed insights into the 3D structure and dynamics of peptides.

What Information Can be Obtained Through NMR Technology?

NMR experiments can be used to study the structural information of peptides, including folding, biomolecular interactions, and spatial structure information, as shown in Figure 1.

Figure 1. NMR spectrum and structure of gramicidin M (Cotton, M.; et al. 1999)

• Creative Proteomics uses one-dimensional hydrogen spectroscopy and two-dimensional homonuclear hydrogen spectroscopy to understand the overall folding of peptides and whether the sample is aggregated without labeling.
• The two-dimensional heteronuclear experiment of Creative Proteomics can be used to study the interaction between peptides and ligands. For the case where the main chain is assigned, we can directly measure the action site of the peptide molecule. For the peptide without the main chain assigned, we can It is possible to detect whether a peptide interacts with a ligand.
• The three-dimensional triple resonance experiment of Creative Proteomics is mainly used for the chemical shift assignment of the main and side chains. We use the three-dimensional nuclear overhauser enhancement (NOE) experiment to obtain spatial structure information of the sample, and combine the main and side chain chemical shift assignments to perform three-dimensional structure calculations.

Requirements of Peptide Samples for NMR Experiments

Peptide Molecular Weight

Generally speaking, the molecular weight of peptides and protein molecules used for NMR detection needs to be less than 30 kDa, the concentration should be greater than 0.5 mM, and the volume should be 450 μL-500 μL.

Buffer

• When preparing samples for nuclear magnetic resonance (NMR) analysis, the phosphate-buffered saline (PBS) system typically works well.
• The total molarity of salt ions in the buffer should remain below 300mM to avoid signal broadening.
• If amino hydrogen observations are desired, maintaining a pH lower than 7.5 is generally recommended.
• Be sure to also include approximately 5% deuterium oxide (D2O, or "heavy water") to facilitate field locking and shimming functions on the spectrometer.

Isotope Labeling

For 1D H-1 NMR spectroscopy and 2D homonuclear H-H correlation experiments, isotopic labeling of peptide samples is unnecessary - these can be run naturally. However, 2D and 3D heteronuclear experiments observing cross-peaks between H-C and H-N nuclei do require isotopic enrichment of carbon-13 and/or nitrogen-15. With labeling, our powerful techniques can provide additional distance and connectivity information beyond what 1D/2D proton experiments alone can reveal.

Highlights of Our NMR-Based Peptide Structure Analysis Services

State-of-the-art NMR technology

Our state-of-the-art equipment enables precise, detailed characterization of peptide structures, including sequence determination, conformational analysis and identification of secondary structure elements.

Comprehensive Data Interpretation

Creative Proteomics provides not just raw NMR data, but comprehensive data interpretation. Our experts analyze NMR spectra, interpret the results and generate detailed reports.

Confidentiality and Data Security

We consider and care about confidentiality and data security. Your peptide samples and data will be handled with the utmost care and protected by strict security measures. We maintain strict protocols to ensure the integrity and confidentiality of your project.

At Creative Proteomics, we are committed to providing comprehensive and reliable peptide structure analysis services. Contact us today to discuss your specific needs and learn how our expertise in NMR-based analysis can advance your peptide research, drug discovery, and development programs.

* For research use only. Not for use in diagnostic procedures!

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