Peptides play a vital role in physiological functions such as blood sugar regulation, nerve conduction, and metabolism. Therefore, peptide identification is of great significance in the fields of drug discovery, immunology research, biological research, and food industry research.
What is Peptide Identification?
Peptide identification mainly refers to the use of physical, chemical, biological and other methods to measure and identify the molecular weight, amino acid composition, sequence, three-dimensional structure and post-translational modifications of peptides to determine the type and nature of the peptide.
How to Identify Peptides?
Peptide Molecular Mass Determination
At Creative Proteomics, we primarily use mass spectrometry and gel electrophoresis to analyze the molecular weight of peptides. Among them, mass spectrometry is the most direct and accurate method to determine the molecular weight of peptides. Based on our advanced mass spectrometry platform, we can determine peptides with a molecular weight range of 0.1-10 kD.
Peptide Amino Acid Composition and Sequence Analysis
We mainly use mass spectrometry to analyze the amino acid sequence of peptides.
We have advanced circular dichroism spectroscopy, mass spectrometry, and nuclear magnetic resonance technology platforms that can analyze the secondary structure, tertiary structure, disulfide bond status and disulfide bond quantitative analysis of peptides in various samples to achieve the purpose of identifying peptides.
Creative Proteomics has extensive experience in the field of peptide PTMs analysis. Our immunological methods can be used to detect modifications such as glycosylation and phosphorylation in peptides, while mass spectrometry methods can be used to determine the type and number of modified amino acids.
Our MS-based Peptide Identification Service Process
Creative Proteomics has a cutting-edge technology platform and a professional technical team. Creative Proteomics, a company with over 20 years’ experience in peptidomics research, offers professional services of peptide identification. Please specify the purpose of your experiment and supply us with the samples, and we will conduct a subsequent experiment for you.
Peptide Identification Service Process
Mass Spectrometry-Based Peptide Sequence Analysis
Peptide identification using mass spectrometry is a powerful technique in peptidomics research. Using matrix assisted laser desorption ionization mass spectrometry (MALDI-MS), electrospray ionization mass spectrometry (ESI-MS) and liquid chromatography with mass spectrometry detection (LC-MS), Creative Proteomics offers high-resolution, high-sensitivity peptide sequencing services and peptide de novo de novo sequencing services utilizing mass spectrometry.
Database Search
In the field of peptide mass spectrometry identification, our biotechnology experts have extensive experience and unique advantages. In accordance with the source of the sample species you supply, we will download the peptide group data from a variety of databases, including NCBI and UniProt. We will match the mass spectrometry data with the database information in order to identify the peptides in the sample using professional software.
Report
After completing the mass analysis, a detailed report containing the following information will be provided:
- Sample preparation methods
- Mass spectrometry parameters and conditions
- MS chromatograms
- Expert interpretation and conclusions
Advantages of Our Peptide Identification Services
- Wide range of sample applications: including peptides in cells, tissues, body fluids, recombinant peptides, synthetic peptides, etc.
- High identification accuracy: Using multiple identification methods, peptides can be identified more accurately.
- Comprehensive peptide identification information: One-stop access to peptide sequence, structure, PTMs and other information.
- Highly automated: Highly automated instruments and equipment are used to automate the separation, purification, detection and data analysis of peptide samples.
Backed by advanced technologies and capabilities, Peptide Identification services from Creative Proteomics support our customers' peptidomics research with accurate and reliable results. Please contact us to learn how we can help advance your scientific discoveries.
* For research use only. Not for use in diagnostic procedures!
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