Creative Proteomics is a leading provider of cutting-edge immunopeptide affinity screening services. Our team of experts employs advanced analysis methods and state-of-the-art instrumentation to identify high-affinity immunopeptides for a wide range of applications. With our comprehensive services, we aim to assist researchers and industries in utilizing immunopeptides to enhance diagnostics, therapeutics, and other biotechnological applications. Our immunopeptide affinity screening service offers accurate and reliable identification of immunopeptides with exceptional binding properties, enabling clients to make breakthrough discoveries and drive innovation in their respective fields.
What is Immunopeptide Affinity Screening?
Peptide screening methods based on peptide exposure on coat proteins generated by fusion of E. coli phage constructs are still under development. Immunopeptide affinity screening is a technique employed to ascertain and extract peptides capable of binding to distinct target proteins or molecules of interest. This method encompasses the generation of a repertoire of peptides, often exhibited on phage or alternative display systems, subsequently subjecting them to scrutiny for their capacity to bind to a specific antibody or receptor. Immunopeptide affinity screening represents a versatile instrument with a multitude of applications, including the domains of antibody exploration, vaccine advancement, diagnostics, targeted therapeutics, investigations into protein-protein interactions, and identification of biomarkers.
Immunopeptide Affinity Screening Services by Creative Proteomics
- Library Screening
- Screening of large peptide libraries against target antigens to identify high-affinity immunopeptides.
- Selection and validation of immunopeptides with optimal binding affinity and specificity.
- Tailored screening strategies based on project requirements and objectives.
- Comprehensive analysis of screening results and peptide ranking.
- Yeast Display
- Employing yeast surface display libraries for high-throughput screening of immunopeptides.
- Display of peptide sequences on the surface of yeast cells, enabling selection based on binding to targets.
- Utilization of fluorescence-activated cell sorting (FACS) for efficient isolation of high-affinity binder populations.
- Optimization and characterization of selected immunopeptides
- In Vitro Selection
- Application of in vitro selection methods, such as RNA aptamers or Ribosome Display, for immunopeptide screening.
- Iterative cycles of selection, amplification, and enrichment to isolate immunopeptides with desired affinity and specificity.
- Rapid identification and characterization of diverse and unique immunopeptides.
- Amenable to a broad range of target antigens, including small molecules, proteins, and membranes.
Our Affinity Screening Platform
Our affinity screening services offer the chance to utilize an exclusive technology platform. This capability provides substantial advantages in terms of throughput, surpassing more conventional screening methodologies like phage display or ribosome display. Moreover, along with screening for soluble targets, the platform can be synergistically combined with other in-house capabilities to enable the unparalleled processing of cell surface targets in primary peptide screens. This ability proves particularly valuable in the identification of target peptides.
- Capture-based kinetics and affinity: screen up to 1152 ligands
- Coupling dynamics: up to 384 fixed ligands
- Epitope binning: interrogating up to 384×384 pairwise competitive interactions
- Quantitative: measure the concentration of up to 1152 samples
- Peptide Mapping: Screen up to 384 analytes against arrays of up to 384 peptides
- Blocking/neutralization assay: 384 assay capacity
- Regenerative Scouting: Parallel Scouting 96 Conditions
- General multiplexing: array any combination of up to 384 ligands
Applications of Affinity Screening
- Antibody discovery: Immunopeptide affinity screening facilitates the discovery of peptides or proteins with a profound affinity for specific antibodies. This advancement aids in the exploration and formulation of novel therapeutic antibodies for a multitude of ailments.
- Vaccine development: Immunopeptide affinity screening enables the identification of peptides or proteins that exhibit robust binding to antigens. These molecules hold great potential as vaccine candidates, capable of provoking targeted immune responses against infectious diseases or cancer.
- Diagnostic tools: Immunopeptide affinity screening plays a pivotal role in the identification of peptides or proteins that specifically bind to disease-associated antigens. These specialized molecules can be employed in diagnostic assays to accurately detect and quantify the presence of the target antigen in patient samples.
Sample Requirement
- Experimental samples will not be returned, please back them up by yourself.
- Before sampling, a control group and an experimental group should be set up, and it is recommended to repeat more than 3 times for each group.
- There is no limit to the variety of peptides.
- For breeds with special requirements or peptides, please contact our staff for more information.
Creative Proteomics is a company with an international customer base. We also work with universities, research institutes, small companies, and start-ups. We offer a range of peptide testing and analysis services. If necessary, please contact us.
* For research use only. Not for use in diagnostic procedures!
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