Using advanced mass spectrometry techniques and bioinformatics analysis, Creative Proteomics provides precise insights into peptide composition, PTMs, and structural variations. Our de novo peptide sequencing service offers accurate and comprehensive determination of peptide sequences without relying on reference databases. Our experienced scientists and cutting-edge technology ensure reliable results tailored to your research needs.
What is Peptide De Novo Sequencing?
De novo sequencing is a powerful technique used to determine the amino acid sequence of a peptide, without relying on a reference sequence database. It involves analyzing MS data, specifically tandem MS/MS, to decipher the sequence based on fragmentation patterns and mass differences between ions.
How Do We Perform Peptide De Novo Sequencing?
Creative Proteomics specializes in providing comprehensive peptide sequencing services. Our experienced scientists are trained in the latest techniques, ensuring that our customers receive accurate and reliable results.
We accept a wide range of sample types, including peptides, proteins, and other biomolecules. Our experienced scientists can guide you in preparing your samples to ensure optimal conditions for sequencing. If you choose to provide samples yourself, please ensure the quality of the samples:
Normal amount: 500 μg
Minimum amount: 200 μg
MS Analysis
The samples undergo tandem MS analysis, where they are fragmented to generate a series of ions. The resulting mass spectra are carefully analyzed to identify ion series and mass differences, enabling the determination of amino acid sequences.
With the power of FTICR-MS, we directly analyze intact peptides and peptides without prior digestion.
- Intact peptide molecular ions are introduced into the mass analyzer.
- Proteins are cleaved into peptide fragments through gas phase lysis.
- The obtained peptide segments were analyzed to determine the amino acid sequence of each peptide segment.
- All peptide sequences are spliced together to obtain the complete protein sequence.
Validation and Verification
The obtained peptide sequence is rigorously validated through internal quality control measures and independent validation methods to ensure accuracy and reliability.
What Can Our Peptide Sequencing Services be Used for?
Identification of Novel Peptides
For peptides absent in existing databases, our sequencing technology enables the discovery of new biomarkers, active peptides, and therapeutic targets.
Identification of Peptide PTMs
Peptide de novo sequencing allows us to identify and characterize PTMs in peptides, providing insights into their functional implications and disease relevance.
Characterization of Protein Variants
Our services enable the characterization of peptide variants, helping researchers understand the diversity of peptide and protein structures and functions.
Advantages of Our Peptide Sequencing Services
- Novelty: Identify proteins not present in existing databases.
- Comprehensive sequence determination: Provide complete protein sequence determination.
- Flexibility and versatility: Suitable for a variety of sample types and can be customized to specific research needs.
- Overcome database limitations: Not limited by database coverage.
- Validation and Verification: Existing annotations can be confirmed or corrected.
Creative Proteomics offers comprehensive peptide de novo sequencing services that provide accurate sequence determination, customized approaches, and a wide range of applications. By choosing our services, you gain access to expert scientists, advanced technology, and reliable results for your research needs. Contact us today to learn more about how our services can benefit your research.
* For research use only. Not for use in diagnostic procedures!
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