At Creative Proteomics, we provide comprehensive peptidomics analysis services with isotope labeling to aid researchers in acquiring invaluable insights into the peptidome, unveiling alterations in patterns of peptide expression, and comprehending the significance of peptides in diverse biological processes. Our team of scientists proficiently utilizes cutting-edge technologies and implements exacting quality control measures to guarantee the precision and dependability of outcomes for each undertaking.
What is Isotope Labeling Peptidomics Analysis Service?
Isotope labeling peptidomics analysis is a powerful analytical technique that focuses on the identification and quantification of peptides in biological samples. It involves the use of stable isotopes, such as 13C and 15N, to differentially label peptides of interest. These labeled peptides are then analyzed using cutting-edge mass spectrometry techniques to determine their sequence and quantify their abundance.
Isotope Labeling Peptidomics Analysis Services by Creative Proteomics
- Peptide Identification
We employ sophisticated mass spectrometry methodologies to identify peptides within intricate samples, furnishing precise data on their sequences and modifications.
- Quantitative Analysis
Through our precise isotope labeling peptidomics analysis, we provide an accurate measurement of peptide quantities, enabling the identification of peptides with differential expression in various biological samples.
- Post-translational Modification Analysis
We specialize in examining post-translational modifications (PTMs) occurring in peptides, encompassing phosphorylation, acetylation, methylation, glycosylation, and more. Our services aid in comprehending the functional implications of PTMs across diverse biological processes.
- Peptide Profiling
Our proficient team conducts all-encompassing peptide profiling to obtain a comprehensive understanding of the peptidome within a specific biological sample. This invaluable information aids in characterizing disease-related biomarkers and facilitates the discovery of new biomarkers.
- Isotope Labeling-based Quantification
Our company excels in the field of isotope labeling methodologies, employing cutting-edge techniques like stable isotope labeling with amino acids in cell culture (SILAC), isobaric tags for relative and absolute quantification (iTRAQ/TMT), and metabolic labeling for accurate quantification purposes.
- Peptide Stability and Degradation Study
We offer comprehensive analyses aimed at studying the stability and degradation patterns of peptides under diverse conditions or treatments. This investigation aids in unraveling the intricacies of peptide half-life, degradation pathways, and potential therapeutic implications.
Isotope Labeling
In isotope-labeled peptidomics analysis, commonly used isotopes include deuterium (D), 13C, 15N and 18O.
Process of Isotope Labeling Peptidomics Analysis
- Sample Preparation: The biological specimen, including cellular or tissue components, is collected and processed in order to extract the peptides of interest.
- Isotope Labeling: The extracted peptides undergo labeling with isotopically enriched amino acids via the chosen labeling methodology.
- Peptide Fractionation: To reduce sample complexity and enhance the detection of peptides present in lower quantities, the labeled peptides may undergo fractionation techniques such as liquid chromatography-based separation.
- Mass Spectrometry Analysis: The labeled peptides are subjected to mass spectrometry analysis, enabling the identification and quantification of peptides within the sample.
- Data Analysis: The obtained data is computationally evaluated using advanced bioinformatics tools and databases, enabling the identification of peptides, determination of their abundance, and analysis of any modifications that may be present.
Advantages of Isotope Labeling Peptidomics Analysis
- Accurate Quantification: Isotope labeling techniques facilitate utmost precision in the quantification of peptides, thereby enabling meticulous determination of the disparateness in expression levels between samples.
- Multiplexing Capability: Employing multiplexing methods such as SILAC and iTRAQ, numerous samples can be concurrently scrutinized, thus optimizing time management and minimizing experimental divergence.
- Comprehensive PTM Analysis: The utilization of isotope labeling peptidomics analysis confers the opportunity to discern and elucidate diverse post-translational modifications, bestowing valuable insights into their pivotal roles in biological processes.
- Biomarker Discovery: Through the profiling of the peptidome, it becomes plausible to unearth potential biomarkers relevant to illnesses, which in turn facilitates the formulation of diagnostic and therapeutic strategies.
Applications of Isotope Labeling Peptidomics Analysis
- Disease Biomarker Exploration: Employing isotope labeling peptidomics analysis allows for the discovery of potential disease biomarkers through the examination of the peptidome and identification of peptides with differential expression.
- Pharmaceutical Advancement: Comprehending the peptidome and post-translational modifications can expedite the development of specific therapies and personalized medical strategies.
- Examination of Protein-Protein Interactions: The fusion of isotope labeling techniques with other methodologies facilitates the exploration of protein-protein interactions, uncovering knowledge about cellular signaling pathways and protein functionality.
Creative Proteomics is a renowned provider of advanced proteomics services, specializing in cutting-edge techniques for comprehensive peptide and protein analysis. Our isotope labeling peptidomics analysis services offer a powerful approach to unraveling the complexity of biological systems and facilitating breakthrough discoveries. If necessary, please contact us.
* For research use only. Not for use in diagnostic procedures!
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