Welcome to Creative Proteomics, your definitive partner in cutting-edge quantitative peptidomics services. Through our tailored services and advanced technological infrastructure, we ensure comprehensive and accurate analysis to meet your scientific needs. analyzing these peptides provides critical insights into various biological processes, including bioactivity, signaling pathways, and potential biomarker discovery. Creative Proteomics offers an extensive array of services designed to cater to the diverse needs of researchers and scientists across the globe.
What is Quantitative Peptidomics?
Quantitative peptidomics constitutes a highly intricate domain of proteomics that aspires to comprehensively investigate and gauge the presence and profusion of peptides within a biological specimen. Peptides, being succinct assemblages of amino acids, play indispensable roles in biological mechanisms such as intercellular communication, neurotransmission, and immune responses. The realm of quantitative peptidomics encompasses the meticulous procedures of peptide identification, characterization, and quantification through the utilization of state-of-the-art mass spectrometry-based methodologies. These methodologies conventionally encompass the enzymatic degradation of proteins to yield peptides, subsequent separation, fragmentation, and ultimately, mass spectrometry analysis. The resultant data engenders profound insights into the relative abundance of peptides across diverse samples or conditions, thereby unveiling potential biomarkers or targets for extensive scientific inquiry.
Quantitative Peptidomics Service by Creative Proteomics
- Label-Free Peptide Quantification Service
We provide peptide quantification. Our team can precisely determine the quantity of your peptide. A variety of methods can be used to quantify peptides.
- iTRAQ-Based Peptidomics Analysis Service
The peptide fragments are labeled with iTRAQ reagents. These reagents contain unique isobaric tags that enable multiplexing of samples. Each iTRAQ label has a specific mass that allows for relative quantification of peptides from different samples.
- SILAC-Based Peptidomics Analysis Service
We offer a service of direct comparative analysis of peptide expression utilizing SILAC methodology, providing the means to discern differences in expression levels among various samples or experimental conditions.
- TMT-Based Peptidomics Analysis Service
Our experts employ TMT reagents to label peptides from different samples with distinct mass tags, facilitating multiplexed analysis.
- Absolute Quantification Service for Peptide
To enable absolute quantification, we can synthesize stable isotope-labeled peptides that serve as internal standards. These labeled peptides are chemically identical to the target peptides but differ in their isotopic composition, allowing for accurate quantification.
- Semi-Quantitative Peptidomics Analysis Service
Semi-quantitative peptidomics analysis allows for the comparison of peptide levels across different samples, providing insights into differences in peptide abundance between experimental conditions.
- PRM-Based Peptide Quantification Service
Our team of proficient scholars shall collaborate closely with you, seeking an intimate comprehension of your research objectives and experimental prerequisites. Consequently, we shall provide adept guidance in conceiving an exemplary PRM endeavor, custom-tailored to your precise exigencies.
- Shotgun Peptidomics Analysis Services
We employ sophisticated algorithms and software to interpret the experimental spectral data, allowing for accurate peptide identification and validation.
- MRM-Based Peptide Quantification Service
Accurate measurement of peptide concentrations in complex biological samples using MRM-based methods.
- Isotope Labeling Peptidomics Analysis Service
Through our precise isotope labeling peptidomics analysis, we provide an accurate measurement of peptide quantities, enabling the identification of peptides with differential expression in various biological samples,
- Isobaric Labeling Peptidomics Analysis Service
- Incorporation of isobaric labels into peptides
- Optimization of labeling conditions
- Multiplexing of samples for simultaneous analysis
Advantages of Quantitative Peptidomics Services
- Comprehensive coverage
Our services boast unparalleled depth and coverage, enabling the identification and quantification of a wide range of endogenous peptides, including those with low abundance.
- High sensitivity and accuracy
Utilizing cutting-edge mass spectrometry platforms, we ensure high sensitivity and accuracy in identifying and quantifying peptides, even in complex biological matrices.
- Customized solutions
Recognizing the diverse nature of research, we offer customizable solutions tailored to meet specific experimental demands, ensuring optimal outcomes and relevance to your research goals.
- Bioinformatics support
Our dedicated team of bioinformaticians provides robust support in data processing, analysis, and visualization, ensuring that the obtained results are translated into meaningful biological insights.
Our Technical Platform
- Triple Quadrupole Mass Spectrometer
- AB SCIEX QTRAP 6500+
- Thermo Scientific TSQ Quantiva
- Waters Acquity Qda
- High Resolution Mass Spectrometer
- Thermo Scientific Q Exactive Plus
- AB SCIEX TripleTOF 6600
- Waters SYNAPT G2-Si
- Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
- Bruker SolariX XR
- Thermo Scientific Orbitrap Fusion Lumos
- Capillary Electrophoresis Mass Spectrometry
- AB SCIEX CESI 8000
- Agilent 7100 CE
Sample Requirements for Quantitative Peptidomics
| Content |
Requirements |
| Volume |
- Liquid Chromatography Mass Spectrometry (LC-MS): 1-10 uL
- MALDI-TOF mass spectrometry: 1-5 nL
- Capillary electrophoresis mass spectrometry (CE-MS): 10-100 nL
|
| Concentration |
The concentration is usually between ng/mL and μg/mL, higher or lower concentration requirements may be required based on specific projects. |
| Sample Type |
It can be peptide standards, peptides in colloids or solutions, peptides in organisms, etc. |
| Impurity limits |
Impurity limits are determined based on specific analytical requirements, which usually require that the content of other proteins, peptides or compounds in the sample is low and does not interfere with the analytical results. |
Our cutting-edge quantitative peptidomics services at Creative Proteomics position us as pioneers in innovation, providing an exhaustive array of analyses meticulously crafted to unravel the intricacy of endogenous peptides within biological systems. Whether your objective is the discovery of biomarkers, the elucidation of pathways, or the comprehension of bioactivity, our unwavering dedication to excellence and scientific rigor guarantees that your research pursuits are fortified with accurate and dependable peptidomics data. Please contact us today and unlock the potential of peptide analysis.
* For research use only. Not for use in diagnostic procedures!
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