We pride ourselves on the exceptional advantages that our esteemed company offers in the realm of SILAC-based peptidomics analysis. With our cutting-edge facilities and a team of highly experienced and skilled scientists, we guarantee unparalleled quality and precision in our services. SILAC, an acronym for Stable Isotope Labeling by Amino Acids in Cell Culture, is an influential technique that confers the ability to quantitatively analyze peptides using stable isotopes. This innovative method involves the incorporation of isotopically labeled amino acids into the sequential structure of peptides during the process of cell cultivation.
What is SILAC-Based Peptidomics Analysis?
SILAC-based peptidomics analysis, also referred to as stable isotope labeling by amino acids in cell culture-based peptidomics analysis, is a quantitative proteomics technique that unveils the ever-changing dynamics and intricate interplay of peptides within a biological system. This sophisticated approach allows for the comparative quantification of peptides amidst diverse samples by incorporating steadfast isotopes into the proteome during cell cultivation. In the course of SILAC-based peptidomics analysis, cells are nurtured in growth media supplemented with either unmarked (light) or isotopically enriched (heavy) amino acids. These heavier amino acids, enriched with stable isotopes like 13C or 15N, supplant the naturally occurring isotopes in the amino acid composition. As the cells assimilate these amino acids, they incorporate them during the synthesis of nascent peptides and proteins. SILAC-based peptidomics analysis has emerged as a potent instrument in deciphering proteolytic processing, unveiling peptide secretion mechanisms, and unraveling peptide-protein interactions. Its diverse applications span across various fields, encompassing biomarker exploration, identification of potential drug targets, and enhanced comprehension of intricate cellular signaling pathways.
SILAC-Based Peptidomics Analysis Services by Creative Proteomics
- Accurate Quantification
Our team allows for precise and reliable quantification of peptides by comparing the intensities of labeled and unlabeled peptide ions in mass spectrometry analysis.
- Comparative Analysis
We offer a service of direct comparative analysis of peptide expression utilizing SILAC methodology, providing the means to discern differences in expression levels among various samples or experimental conditions. This approach proves particularly valuable in the identification of differentially expressed peptides and the elucidation of their roles within biological processes.
- Dynamic Proteomics
SILAC-Based Peptidomics Analysis can be employed to investigate changes in peptide abundance over a temporal dimension. By employing SILAC labeling at distinct time points, researchers are able to monitor the dynamics of peptide expression, thereby gaining valuable insights into protein synthesis, degradation, and turnover.
- Sample Preparation
We provide comprehensive support in the preparation of samples for analysis, ensuring meticulous processing and optimization for peptidomics investigation.
- LC-MS/MS Analysis
Our cutting-edge liquid chromatography-tandem mass spectrometry (LC-MS/MS) platforms allow for precise and sensitive measurement of peptide levels. We harness advanced instrumentation and optimized methodologies to ensure the acquisition of dependable and high-quality data.
Analysis Techniques and Instrumentation of SILAC-Based Peptidomics Analysis
- Liquid Chromatography (LC): We employ high-resolution LC separation to achieve efficient sample preparation and separation of peptides prior to mass spectrometry analysis.
Instrument model: Agilent 1290 Infinity II LC System
- Tandem Mass Spectrometry (MS/MS): Our advanced MS/MS platforms allow for accurate peptide sequencing and quantification, providing high-resolution and sensitive analysis of peptidomic samples.
Instrument model: Thermo Fisher-Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Our SILAC-Based Peptidomics Analysis Process
- Consultation: Our team of esteemed scholars will engage in a scholarly discussion with you to explore your research objectives, sample properties, and specific prerequisites.
- Sample Submission: Conveniently deliver your samples to our esteemed laboratory via our secure and efficient logistics network.
- Sample Preparation: We ensure the expeditious and dependable preparation of your samples, encompassing the extraction of proteins, their digestion, and the meticulous SILAC labeling process for precise quantification.
- LC-MS/MS Analysis: Peptides are efficiently separated via liquid chromatography and meticulously scrutinized through tandem mass spectrometry, culminating in the generation of comprehensive peptidomic profiles.
- Data Processing and Analysis: Our accomplished team of bioinformatics experts employs cutting-edge algorithms and software to seamlessly process and dissect the acquired data, yielding exhaustive reports replete with peptide identification and relative quantification outcomes.
- Statistical Analysis: We conduct rigorous statistical analyses to identify peptides displaying noteworthy changes in abundance, thereby unraveling their profound biological implications.
Advantages of SILAC-Based Peptidomics Analysis
Integration with Proteomic Studies: SILAC can be amalgamated with additional proteomic methodologies, including mass spectrometry-dependent protein identification and characterization, in order to confer an all-encompassing comprehension of the proteome and its corresponding peptides.
Why Choose Us?
- Comprehensive Peptide Identification: Our semi-quantitative peptidomics analysis facilitates the elucidation of a myriad of peptides in intricate biological samples, unveiling both established and innovative peptides.
- Relative Quantification: By employing label-free quantification techniques, we can juxtapose the relative abundance of peptides across diverse samples or experimental circumstances, bestowing us with discernment into alterations in the peptidome.
- Biomarker Discovery: Our statistical analysis empowers the discernment of peptides that exhibit noteworthy alterations in abundance, harboring the potential to serve as biomarkers for specific biological processes, diseases, or therapeutic interventions.
- Deep Profiling: Our semi-quantitative peptidomics analysis enables an in-depth profiling of the peptidome, endowing us with a comprehensive comprehension of the composition and dynamics of peptides in biological samples.
* For research use only. Not for use in diagnostic procedures!
Our customer service representatives are available 24 hours a day, 7 days a week.
