At Creative Proteomics, our shotgun peptidomics analysis services utilize state-of-the-art mass spectrometry technologies and advanced bioinformatics analyses to identify and quantify peptides across a wide range of biological samples. Whether you are studying peptide signaling pathways, investigating disease biomarkers, or exploring peptide-based therapeutics, our services can support your research needs.
What is Shotgun Peptidomics Analysis?
Shotgun peptidomics analysis is an expedient proteomic technique employed for exhaustive profiling and characterization of peptides in biological specimens. It encompasses enzymatic hydrolysis of proteins into peptides, followed by mass spectrometry-based interrogation to detect and quantify the peptides within. Diverging from targeted peptidomics analysis, wherein only specific peptides of interest are selectively scrutinized, shotgun peptidomics permits an open-ended and comprehensively unbiased exploration of the peptide constituents within a given sample. It enables the identification of both established and innovative peptides, thus enlightening us about the intricacies and heterogeneity of the peptide interplay within the sample. The extensive applicability of shotgun peptidomics analysis spans diverse fields encompassing biomedical research, pharmaceutical drug exploration, and biomarker discovery. It affords us the ability to investigate the dynamics of peptide involvement in biological processes, pinpoint peptide-based biomarkers for disease diagnosis or monitoring, and delve into peptide modifications or post-translational alterations.
Shotgun Peptidomics Analysis Services by Creative Proteomics
- Spectrum Interpretation
We employ sophisticated algorithms and software to interpret the experimental spectral data, allowing for accurate peptide identification and validation.
- Novel Peptide Discovery
In instances where traditional databases are insufficient, or for the exploration of undiscovered peptides, we utilize innovative de novo sequencing algorithms to reconstruct peptide sequences solely from the experimental MS/MS spectra.
- Analysis of Peptide Modifications
Our comprehensive analysis pipeline includes the identification and characterization of post-translational modifications (PTMs), such as phosphorylation, acetylation, methylation, and glycosylation. This enables a deeper understanding of the functional implications of PTMs on peptide activity and regulatory mechanisms.
- Peptide Identification and Elucidation
Through the utilization of cutting-edge databases and advanced bioinformatics tools, we conduct meticulous peptide identification, sequence validation, and characterization, inclusive of analysis on post-translational modifications (PTMs).
- Sample Preparatory Techniques
We offer meticulous sample extraction and purification procedures, employing denaturation, reduction, and alkylation techniques to ensure the provision of pristine samples for accurate and precise peptidomics analysis.
- Peptide Annotation and Characterization
Our platform provides robust annotation and characterization tools that aid in the identification of post-translational modifications, such as phosphorylation or glycosylation, within the identified peptides. Users can effortlessly search for known peptides or motifs of interest.
Technological Advantages
- Quantitative Analysis: Our shotgun peptidomics analysis services provide semi-quantitative information on peptide abundance. By employing label-free quantification methods, we can compare peptide levels across different samples or conditions, enabling the identification of differentially expressed peptides.
- High Sensitivity: Our shotgun peptidomics analysis platform enables the detection of low-abundance peptides present in biological samples.
- Comprehensive Coverage: The shotgun peptidomics approach allows for the comprehensive analysis of the peptidome, ensuring that a wide range of peptides are detected and quantified. This includes both known and novel peptides, providing valuable insights into the peptide landscape of a sample.
- Identification of Post-translational Modifications (PTMs): Shotgun peptidomics analysis allows for the identification of peptides with PTMs, such as phosphorylation, acetylation, or glycosylation. This information provides important insights into the functional and regulatory roles of peptides in biological systems.
Our Platform for Shotgun Peptidomics Analysis
- Triple quadrupole mass spectrometer: such as AB SCIEX TripleTOF 6600, Thermo Scientific TSQ Quantiva, Waters Xevo TQ-XS, etc.
- Orbitrap mass spectrometer: such as Thermo Scientific Q Exactive, Grigorovici EMR, Bruker MaXis 4G, etc.
- Ion mobility mass spectrometer: such as Waters SYNAPT G2-Si, Agilent 6560 IM-QTOF, etc.
- TOF/MS mass spectrometer: such as Sciex TripleTOF 5600, Bruker Daltonics ultrafleXtreme, etc.
Customer Notice
- Sample source: It can be body tissues or cells, medical samples (such as blood, urine, etc.), bacterial or fungal cultures, etc.
- Sample pretreatment: Samples require appropriate extraction, purification, and pretreatment steps to isolate the protein or peptide of interest.
- Sample quality: Samples should be of sufficient purity and concentration to ensure reliable results during analysis.
- Sample complexity: The complexity of the sample may affect the sensitivity and specificity of shotgun peptide analysis. Therefore, it is necessary to consider how to reduce the complexity of the sample during the sample preparation process, such as removing high-abundance proteins, desalting proteins, etc.
- Sample labeling: In some studies, samples may need to be labeled, such as fluorescent labels, isotope labels, etc., to facilitate quantification in mass spectrometry analysis.
Results Delivery
1. Detailed report, including experiment procedures, parameters, etc.
2. Raw data and data analysis results
* For research use only. Not for use in diagnostic procedures!
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