Peptide sample preparation is a critical step in peptidomics research, where targeted peptides are extracted from complex biological samples for downstream analysis using mass spectrometry. The quality of peptide samples directly affects the success of peptidomics research, making peptide sample pre-processing services an essential requirement for accurate and reliable results.
How We Prepare Peptide Samples
Creative Proteomics offers high-quality peptide sample preparation services for researchers in pharmaceutical, biotechnology organizations and academia. Our services cover the entire pipeline of peptide sample preparation, from peptides extraction to peptides purification, and are tailored to meet the specific needs of individual research projects.
Samples Requirements
Creative Proteomics' team is experienced in handling different types of peptide samples. Common samples include cell samples, tissue samples and body fluid samples. In addition, samples for peptidomics research can be gel dots, gel strips, IP or Co-IP samples, paraffin-embedded samples, exosomes, subcellular cells, etc. The table below (Table 1) is some common requirements for your delivered samples. We always provide a free consultation to discuss which workflow is suitable for your research goals before processing.
Requirements for Delivered Samples
| Sample Types |
Sample Preparation Essentials |
Minimum Sample Size |
| Cell |
Keep frozen, avoid freeze-thaw |
Cells number ≥5×106 or cells pellet ≥30 pL |
| Bacteria/Fungi |
Avoid protein contamination in the media |
≥ 200 mg/ ≥400 mg |
| Animal tissue |
Strip blood vessels, fat, and remove blood |
Fresh weight ≥100 mg |
| Plant tissue |
Freshly sampled to avoid contamination |
Fresh weight ≥200 mg |
| Serum/Plasma |
Avoid hemolysis or coagulation |
≥500 pL |
| Urine |
Add sodium azide (0.05～0.1% w/v) for antiseptic and sterilization |
≥5 mL |
| Gel strip |
Remove excess glue to avoid contamination |
The strip is visible to the naked eye. |
Peptides Extraction
Peptides extraction is the first and most crucial step in peptide sample preparation. Our company uses a variety of techniques, including mechanical, chemical, and physical methods, to extract peptides from complex biological samples such as blood plasma, serum, urine, CSF, and synovial fluid. We optimize peptide extraction conditions to ensure high yield and purity of peptides, minimizing the loss of target peptides.
Peptides Digestion
Peptides digestion is the next step in peptide sample preparation, which plays a key role in peptide sample preparation and is a critical intermediate step between raw peptide samples and downstream mass spectrometry analysis. With rich experience in sample preparation, Creative Proteomics will select appropriate enzymes and digestion conditions based on the samples provided by customers, and carefully optimize factors such as pH, temperature, and digestion time, while maintaining the stability and integrity of the target peptide.
Peptides Purification
Peptide digestion results in a crude sample, and the mixture at this point often contains impurities such as unreacted enzymes, buffer components, and other contaminants. The samples are therefore further purified and analyzed for peptide purity. Creative Proteomics uses a comprehensive purification strategy that includes techniques such as dialysis, affinity chromatography, size exclusion chromatography, ion exchange chromatography, and SDS-PAGE. Each purification technique is carefully selected and optimized to effectively remove impurities while minimizing the loss of target peptides.
Advantages of Our Peptide Sample Preparation Services
Creative Proteomics offers comprehensive expertise and services in peptide sample preparation, ensuring the generation of high-quality peptides suitable for downstream analysis.
- State-of-the-art facilities
- Experienced scientists
- Customized services
- Fast turnaround time
Creative Proteomics offers high-quality and reproducible peptide sample preparation services tailored to meet individual research projects' specific needs. You only need to specify your experimental purpose and deliver the experimental samples to us, and we will handle the subsequent experiments. If you have questions or special requirements about our sample preparation services, please contact us!
* For research use only. Not for use in diagnostic procedures!
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