Creative Proteomics is your trusted partner in human leukocyte antigen peptide analysis. Our cutting-edge service is designed to provide comprehensive and accurate analysis of human leukocyte antigen (HLA) peptides, allowing for a better understanding of immune responses and personalized medicine. With our expertise and state-of-the-art technologies, we deliver reliable results to drive your research forward and empower advancements in immunology.
What is Identification of HLA Peptides?
The antigenicity of a cell is delineated by the peptides to which its HLA molecules bind. Through this antigen presentation, T cell-specific responses are controlled. Since a portion of the expressed mutant peptides are presented on HLA, these neoepitopes may be immunogenic. Breakthroughs in HLA peptidomics have greatly expanded the database of natural HLA ligands and enabled detailed characterization of HLA peptide binding specificities
HLA Peptides Analysis by Creative Proteomics
- HLA Peptide Profiling
Our services encompass a thorough analysis of HLA peptides to identify and characterize their constitution in specific individuals. Through implementing cutting-edge methodologies, we ascertain the distinctive repertoire of HLA peptides, thereby facilitating comprehension of an individual's immune response and the potential manifestation of diseases.
- HLA-II peptidomics analysis
- HLA-I peptidomics analysis
- HLA Peptide Binding Assays
We perform binding assays to evaluate the intricate interactions between molecules and HLA peptides
- Neoantigen Identification
Our company excels in neoantigen identification by analyzing HLA peptides derived from tumor tissues. This information aids in designing personalized cancer immunotherapies, as neoantigens can be targeted specifically to enhance anti-tumor immune responses.
- Vaccine Design
We assist in vaccine design by identifying HLA-binding peptides from pathogens or tumor antigens. Our analysis helps in selecting the most immunogenic peptides that can be used to develop effective vaccines, targeting specific HLA allelic populations.
- HLA Typing
We offer accurate HLA typing services using state-of-the-art techniques, including next-generation sequencing (NGS). HLA typing is essential for determining tissue compatibility in transplantation, predicting disease susceptibility, and understanding autoimmune disorders.
- Polymerase chain reaction (PCR)
- Sequence-specific oligonucleotide probes (SSOP)
- Sequencing
- HLA Peptide Elution
Recovering and analyzing molecules bound to HLA peptides to identify potential immunogenic epitopes.
- HLA Epitope Analysis
Identifying specific regions of HLA molecules that interact with T-cell receptors and evaluating their potential role in immune response.
- LC-MS/MS Analysis of HLA Peptidome
Techniques and Instrumentation for HLA Peptide Analysis
- PCR-based Systems
- Applied Biosystems 3500/3730 DNA Analyzers
- Roche LightCycler instruments
- PCR amplification and sequencing
- Fluorescence-Activated Cell Sorters (FACS)
- BD FACSCanto
- Beckman Coulter CytoFLEX
Analysis Methods
At Creative Proteomics, we employ state-of-the-art technologies and analysis methods to ensure the highest quality results:
- Mass Spectrometry: We employ the application of liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) to discern and delineate HLA peptides. This meticulous technique bestows upon us a heightened sense of sensitivity, resolution, and specificity, enabling us to achieve a comprehensive and detailed profiling of peptides.
- Peptide Sequencing: We utilize sophisticated database searching algorithms and innovative de novo sequencing methods to elucidate the precise amino acid sequences of identified HLA peptides. This invaluable information is fundamental for the intricate process of epitope mapping and gaining a comprehensive understanding of immune responses.
Why Choose Us?
- Custom Solutions: Our organization offers customized solutions tailored to individual needs, whether it be HLA typing for graft compatibility, studies on disease associations, or mapping of peptide epitopes.
- Exhaustive Analysis: Our array of services encompasses various facets of HLA peptide analysis, spanning from epitope prediction and determination of allele frequencies to peptide binding assays and beyond.
- Data Precision and Confidentiality: We prioritize the utmost precision in data and uphold strict confidentiality. Our laboratory adheres to stringent quality control measures, ensuring dependable and reproducible results.
- Competitive Pricing: We understand the budgetary constraints faced by researchers and healthcare institutions, and we aim to extend cost-effective solutions.
HLA peptide analysis is important in various fields, including tissue typing for transplantation, disease association studies, and personalized medicine. If necessary, please feel free to contact us.
* For research use only. Not for use in diagnostic procedures!
Our customer service representatives are available 24 hours a day, 7 days a week.
