Introduction
Most biochemical reactions in a cell are regulated by highly specialized proteins, which are the prime mediators of
the cellular phenotype. Therefore the identification, quantitation and characterization of all proteins in a cell
are of utmost importance to understand the molecular processes that mediate cellular physiology. Proteomics is a
large-scale comprehensive study of a specific proteome, including information on protein abundances, the variations
and modifications, as well as their interacting partners and networks. We can find some "disease-specific
protein molecules" through the comparative analysis of the proteome between normal individuals and pathological
individuals, which can become molecular targets for new drug design, or also provide molecular markers for early
diagnosis of diseases. And that mass spectrometry (MS)-based proteomics is the most comprehensive approach in the
most analytical methodsin for the quantitative profiling of proteins, their interactions and modifications.
Fig. 1. Some common
applications of MS-based proteomics in biology. (Sinha, et al., 2020)
Our Services
At Creative Proteomics, we offer two different mass spectrometry strategies to accelerate your
proteomics research, top-down and bottom-up respectively. In the top-down strategy, the intact protein, isn't
degradated, is directly analyzed by LC-MS, and its sequence is determined according to the fragment ions in the
spectrum. while in the bottom-up strategy, the intact protein is first hydrolyzed to mixture into peptides by
protease digestion. Then, the peptides were detected by LC-MS, and the peptides were de novo sequenced to obtain the
complete protein sequence.
Our mass spectrometry (MS)-based proteomic analysis is an attractive and innovative practice that identifies proteins
with extreme precision and sensitivity. In current technology, mass spectrometry methods combined with liquid
chromatography help to quantify the expression of thousands of proteins and facilitate the analysis of dynamic
proteomic changes that occur under specific conditions. In the top-down strategy, our highly professional scientists
developed another brand-new protein analysis method. Compared with traditional sequencing, our scientists use a
variety of non-specific proteases to continuously digest proteins to improve the types and overlaps of protein
digested peptides, thereby increasing the coverage of protein sequencing.
Creative Proteomics offers the following featured proteomics analysis services to assist your
scientific research with years' experience in advanced experiment equipment.
Creative Proteomics has a dedicated team of bioscientists, consist of researcher with experience in
proteomics, to provide a range of proteomics and bioinformatics analysis services to our clients. Thanks to our
biological expertise and data analytics capabilities, we provide mass spectrometry-based proteomics solutions
capable of simultaneously quantifying thousands of proteins in thousands of samples. Please contact us immediately If you are interested in our services.
References
- Aslam B, Basit M, Nisar M A, et al. (2017) Proteomics: technologies and their applications. J
Chromatogr Sci. 55(2):182-196.
- Sinha, Ankit & Mann, Matthias. (2020) A beginner's guide to mass spectrometry-based proteomics. The
Biochemist. 42.
