Peptide Purity Analysis Service

Peptide Purity Analysis Service

Service Details


Peptide purity refers to the amount of correct peptides in a peptide product. The quality and purity of synthetic peptides are typically checked by analytical reversed-phase high-performance chromatography (RP-HPLC), followed by mass spectrometry (MS) with ultraviolet (UV) detection at 210-220 nm. The purity of peptides is a key factor affecting the results of research projects, which has led to the widespread use of peptide purity analysis in various industries. Notably, common contaminants in peptide products come from chemical synthesis, culture media, or incorporation from extraction and purification processes, including peptides with deleted sequences, truncated sequences, incomplete deprotection, and by-products generated during synthesis or final cleavage. To ensure the success of various peptide experiments, it may be necessary to remove trifluoroacetic acid (TFA), or to assess the solubility of hydrophobic peptides prior to performing the desired experiment.

Peptide Purity Analysis Service

Our Services

With our comprehensive peptide analysis platform, Creative Proteomics has developed a highly sensitive and specialized platform. We can provide professional peptide purity determination services using analytical reversed-phase HPLC coupled with mass spectrometry. Each peptide is rigorously monitored during the analytical cycle according to stringent analytical quality assurance and biosynthetic quality control standards.

Throughout the service cycle, our team of experts begins by determining what level of purity you require, or rather, which impurities can be tolerated in your application. The most common impurities include:

  • Delete sequence.
  • Truncated sequences.
  • Incompletely deprotected sequences.
  • Sequences modified during cleavage (reattachment of protecting groups elsewhere in the peptide).
  • Other side reaction products formed during synthesis (e.g. asparagine formation, oxidation products).
  • TFA (Trifluoroacetic Acid).
  • Acetic acid.

Here, our expert team provides the following peptide purity analysis services.

  • Reverse-Phase High Performance Liquid Chromatography

We use advanced reverse-phase HPLC to determine the purity of client peptide samples. In theory, peptide purity is quantified by the chromatogram produced by reversed phase HPLC, and in addition, we can obtain the number and relative amount of possible by-products by calculating the peak area.

  • Mass Spectrometry

In addition to RP HPLC, our talented scientists can also provide information on the molecular weight of peptides determined by mass spectrometry. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and electrospray ionization mass spectrometry (ESI MS) are the two most commonly used methods.

*Note: we can provide amino acid analysis (AAA) to determine the amino acid composition in peptide or protein products, ensuring experimental accuracy and consistency.

What Can We Provide?

  • High quality peptide purity assessment.
  • Certificate of analysis is included with the analytical data.
  • MS and HPLC report files.

Creative Proteomics is a trusted provider of proteomics analysis services. Thanks to our outstanding scientists and powerful mass spectrometry platform, we off provides global customer with comprehensive and professional peptide purity analysis service by using RP-HPLC. If you have any questions or specific requirements, please feel free to contact us. We will fulfill your goal.

For research use only, not intended for any clinical use.

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