Multi-Channel Phosphorylated Protein Analysis Service

Multi-Channel Phosphorylated Protein Analysis Service

Service Details


Protein phosphorylation, a reversible post-translational modification (PTM) in various organisms, plays a key role in the regulation of signal transduction, transcriptional and translational regulation, and the regulation of intracellular biological processes such as metabolism. The study of protein phosphorylation helps to understand life activities and helps to elucidate the mechanism of diseases related to abnormal protein phosphorylation.

Mass spectrometry (MS) is a promising tool for analyzing protein phosphorylation, capable of analyzing thousands of proteins in a single analysis. However, it is necessary to enrich phosphorylated peptides to improve the abundance of modified proteins before quantitative analysis of phosphorylated protein samples by quantitative proteomics analysis due to the low content and wide dynamic range of phosphorylated proteins in biological samples. Currently, a variety of enrichment techniques, including immunoprecipitation (IP), chemical modification, immobilized metal affinity chromatography (IMAC), and metal oxide affinity chromatography (MOAC), have been developed and applied to study phosphorylation of different biological samples.

Fig.1. Different strategies of phosphopeptide enrichment.Fig.1. Different strategies of phosphopeptide enrichment. (Li X S, et al., 2016)

Our Services

With a powerful mass spectrometry sequencing platform and multi-pathway enrichment kit, Creative Proteomics pushs out a multi-pathway phosphorylated proteome analysis platform, a complement to phosphorylated protein analysis, to help you understand phosphorylation more easily.

At Creative Proteomics, our specialists use the multi-pathway enrichment kit to enrich phosphorylated peptides prior to mass spectrometry analysis. Compared with other phosphopeptide enrichment methods, multi-pathway enrichment kit enrichment is easy to operate and enables large-scale analysis of phosphorylated proteins.

With cutting-edge equipment and expertise in protein sequencing and mass spectrometry, we provide one-stop multi-pathway phosphorylated protein analysis service. You only need to tell us the purpose of your experiment and send your samples to us, we will take care of all the follow-up matters of the project. The simple process of our service includes:

  • Protein extraction.
  • Protein digestion.
  • Phosphorylated peptide enrichment (multi-pathway enrichment kit is used).
  • Peptide fragmentation separation.
  • Mass spectrometry analysis.
  • Bioinformatics analysis.

Advantages of Our Services

  • High precision: Targeted screening and quantitative analysis of a defined set of protein sites and signal transduction nodes are allowed.
  • High-throughput: 4000 phosphorylation sites are easily detected.
  • High specificity and sensitivity: Specialized kits for quantitative analysis of post-translational modification (PTM) sites in cellular proteins.
  • Advanced mass spectrometry platform: With high resolution and high scanning speed mass spectrometry, the detection speed is nearly doubled.
  • Fast turnaround time: Full report available in 5-7 days.
  • Customized service: The most efficient and economical solution is customized by our experts according to your requirements.

Sample Requirements

  1. Tissue samples: Plant tissue samples > 200 mg, blood samples > 1 mL (with EDTA for anticoagulation of plasma), serum > 0.5 mL, urine> 2 mL, animal tissue samples > 1 g, cell samples > 5X107 cells, microorganisms > 200 mg (dry weigh).
  2. Protein samples > 1 mg.

Thanks to our powerful mass spectrometry sequencing platform, Creative Proteomics provides a one-stop multi-channel hosphorylated rotein analysis service. Our experienced scientists work with you to develop tailor-made analytical solutions. Please feel free to contact us with any questions regarding multi-channel hosphorylated rotein analysis.


  1. Yang C, Zhong X, Li L. (2014) Recent advances in enrichment and separation strategies for mass spectrometry-based phosphoproteomics. Electrophoresis. 35(24):3418-3429.
  2. Li X S, Yuan B F, Feng Y Q. (2016) Recent advances in phosphopeptide enrichment: Strategies and techniques. TrAC Trends in Analytical Chemistry. 78:70-83.

For research use only, not intended for any clinical use.

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