Protein Full-Length Sequencing

Protein Full-Length Sequencing

Service Details


The analysis process of recombinant protein expression and purification products requires confirmation of the full length of the protein, especially the development and process establishment of recombinant protein products or antibody agents. Determining the complete sequence of the protein is helpful to analyze the structure of the protein and reveal the biological function of the protein. In traditional "bottom-up" proteomic strategy, database searching is used to identify sequences of peptides and proteins analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein sequences absent from the database cannot be identified, and even if present in the database, complete sequence coverage is rarely acheved even for the most abundant proteins in the sample.

Fig. 1. Full-length protein sequencing based on continuous digestion using non-specific proteases.Fig. 1. Full-length protein sequencing based on continuous digestion using non-specific proteases. (Yang C, et al., 2021)

Our Services

Creative Proteomics has developed a full-length protein sequencing method based on continuous digestion using various non-specific proteases by utilizing the existing high-resolution mass spectrometry platform, which chould increase the accuracy and coverage of protein sequencing. A continuous digestion device was constructed, and a variety of non-specific proteases were used to continuously digest the protein. Taking advantage of the non-specific cleavage sites of non-specific proteases, the complementarity of peptides produced at different time and by different kinds of proteases, the type and overlapping degree of digested peptides were improved. The sequence coverage of peptides after continuous digestion by each protease can reach 100%. This analysis can be used to confirm whether the recombinant protein is fully expressed and to detect whether the recombinant protein expression process is broken.

Creative Proteomics has developed the following a customized sequencing service process for protein full-length sequencing analysis services.

Ⅰ. Predicted sequence of a protein based on the gene are analyzed to selecte different enzymes resulting in complement digestive sites.

Ⅱ. Protein are digested separately by selected enzymes.

Ⅲ. The previous step digested pepiteds are analyzed by HPLC-MS/MS.

Ⅳ. MS data are analyzed and pieced together for full-length protein sequence.

Sample requirements

  • Gel samples and solution samples could be used for the above protein full-length sequencing.
  • Amount: 10-20 μg.
  • Purity of protein samples should be as high as possible.

Our Technical Advantages

  • Peptide coverage up to 100%.
  • High sensitivity.
  • A formal report, reporting the experimental process, protein full sequence information, and secondary map support.


  • Determination of Full-Length Sequence of Biologics (Proteins/Peptides/Antibodies/Vaccines)
  • Analysis of PTMs
  • Verification of the Correct Translation of Recombinant Proteins

Please feel free to contact us If you would like to discuss the best way to determine the protein full-length sequencing of your protein. At Creative Proteomics, we not only provide professional protein full-length sequencing service projects for global customers, our scientists can also provide customized services according to your specific requirements, and describe them in detailed project reports, we look forward to with your cooperation.


  1. Yang C, Shan Y C, Zhang W J, et al. (2021) Full-length Protein Sequencing Based on Continuous Digestion Using Non-specific Proteases. Acta Chimica Sinica. 79(5): 663-669.

For research use only, not intended for any clinical use.

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