Introduction
With the successive listing of antibody drugs, accurate sequence determination and structural characterization of
antibodies have become more and more important in the process of drug development. The need for complete
characterization of the quality attributes of these antibodies requires sophisticated techniques. Mass spectrometry
(MS) has become an important analytical tool for the structural characterization of therapeutic antibodies, due to
its superior resolution over other analytical techniques. It has been widely used in virtually all phases of
antibody development. Structural features determined by MS include amino acid sequence, disulfide linkages, and many
different post-translational, in-process, and in-storage modifications.
Fig. 1.
LC-MS versus LC-MS/MS for quantitation of therapeutic MAbs. (Ladwig P M, et al., 2017)
Our Services
Creative Proteomics' deep antibody research knowledge reserve and skilled R&D team, after
years of development, have built a complete antibody sequencing platform and applied mature sequencing technology to
provide customers with one-stop antibody sequencing services. The new generation antibody sequencing platform to
achieve fast and accurate analysis of antibody primary structure, so as to ensure that the every accuracy of
antibody sequencing by using high-quality mass spectrometry platform and full-featured antibody sequencing data
processing software, combined with bioinformatics database. This platform is suitable for sequence analysis of
different subtypes of antibodies (IgG and IgM) and different types of antibodies (fluorescence-conjugated
antibodies, immobilized antibodies, antibodies of different species).
Fig. 2. Workflow of antibody sequencing.
At Creative Proteomics, our mass spectrometry based antibody sequencing services can analyze the
full-length sequence and variable region amino acid sequence of antibodies to obtain complete sequence information
of light and heavy chains of antibodies for our clients. In addition, we also provide hybridoma sequencing services
for our clients.
Creative Proteomics offers the following mass spectrometry-based sequence analysis services.
Advantages of Our Services
- Full Sequence Analysis
The high-resolution mass spectrometry work platform and PCR monoclonal antibody
sequencing service can ensure the full sequence analysis of the entire antibody from the N-terminus to the
C-terminus.
- High Accuracy
The amino acid at each position in the sequence is supported by more than ten different
peptides, ensuring that each amino acid has strong ion fragment peak evidence in MS/MS for deduction.
- Wide Applicability
The technology is applicable to all antibody types (IgG, IgA, IgM, IgY, Fab, ScFv, etc.)
and species (human, mouse, rat, rabbit, hamster, etc.). We can also sequence conjugated and contaminating
proteins and antibodies with two different light chains.
- High Quality Service
Our services maximize the workflow and bioinformatics analysis process to provide you
with the highest quality service.
- Hi-Throughput Detection
Whether you want to sequence just one antibody or hundreds of antibodies, we can do
it.
Creative Proteomics provides comprehensive mass spectrometry based antibody sequencing projects for
global customers. The mass spectrometry platform we developed is capable of providing services of the full-length
sequence of the antibody and the amino acid sequence of the variable region. But if you have any special
requirement, you are welcome to contact us for custom service. We look
forward to your happy cooperation.
References
- Sergey S Z, Sergey V K, Tatiana Y S, et al. (2017) An EThcD-Based Method for Discrimination of Leucine
and Isoleucine Residues in Tryptic Peptides. J Am Soc Mass Spectrom. 28(8):1600-1611.
