Mass Spectrometry Based Antibody Sequencing

Mass Spectrometry Based Antibody Sequencing

Service Details


With the successive listing of antibody drugs, accurate sequence determination and structural characterization of antibodies have become more and more important in the process of drug development. The need for complete characterization of the quality attributes of these antibodies requires sophisticated techniques. Mass spectrometry (MS) has become an important analytical tool for the structural characterization of therapeutic antibodies, due to its superior resolution over other analytical techniques. It has been widely used in virtually all phases of antibody development. Structural features determined by MS include amino acid sequence, disulfide linkages, and many different post-translational, in-process, and in-storage modifications.

Fig. 1. LC-MS versus LC-MS/MS for quantitation of therapeutic MAbs.Fig. 1. LC-MS versus LC-MS/MS for quantitation of therapeutic MAbs. (Ladwig P M, et al., 2017)

Our Services

Creative Proteomics' deep antibody research knowledge reserve and skilled R&D team, after years of development, have built a complete antibody sequencing platform and applied mature sequencing technology to provide customers with one-stop antibody sequencing services. The new generation antibody sequencing platform to achieve fast and accurate analysis of antibody primary structure, so as to ensure that the every accuracy of antibody sequencing by using high-quality mass spectrometry platform and full-featured antibody sequencing data processing software, combined with bioinformatics database. This platform is suitable for sequence analysis of different subtypes of antibodies (IgG and IgM) and different types of antibodies (fluorescence-conjugated antibodies, immobilized antibodies, antibodies of different species).

Workflow of antibody sequencingFig. 2. Workflow of antibody sequencing.

At Creative Proteomics, our mass spectrometry based antibody sequencing services can analyze the full-length sequence and variable region amino acid sequence of antibodies to obtain complete sequence information of light and heavy chains of antibodies for our clients. In addition, we also provide hybridoma sequencing services for our clients.

Creative Proteomics offers the following mass spectrometry-based sequence analysis services.

Advantages of Our Services

  • Full Sequence Analysis
    The high-resolution mass spectrometry work platform and PCR monoclonal antibody sequencing service can ensure the full sequence analysis of the entire antibody from the N-terminus to the C-terminus.
  • High Accuracy
    The amino acid at each position in the sequence is supported by more than ten different peptides, ensuring that each amino acid has strong ion fragment peak evidence in MS/MS for deduction.
  • Wide Applicability
    The technology is applicable to all antibody types (IgG, IgA, IgM, IgY, Fab, ScFv, etc.) and species (human, mouse, rat, rabbit, hamster, etc.). We can also sequence conjugated and contaminating proteins and antibodies with two different light chains.
  • High Quality Service
    Our services maximize the workflow and bioinformatics analysis process to provide you with the highest quality service.
  • Hi-Throughput Detection
    Whether you want to sequence just one antibody or hundreds of antibodies, we can do it.

Creative Proteomics provides comprehensive mass spectrometry based antibody sequencing projects for global customers. The mass spectrometry platform we developed is capable of providing services of the full-length sequence of the antibody and the amino acid sequence of the variable region. But if you have any special requirement, you are welcome to contact us for custom service. We look forward to your happy cooperation.


  1. Sergey S Z, Sergey V K, Tatiana Y S, et al. (2017) An EThcD-Based Method for Discrimination of Leucine and Isoleucine Residues in Tryptic Peptides. J Am Soc Mass Spectrom. 28(8):1600-1611.

For research use only, not intended for any clinical use.

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