Introduction
Full-spectrum protein analysis is to use mass spectrometry technology to identify the full-spectrum protein of
complex biological samples (such as serum, urine, and cell lysates, etc.), and its purpose is to analyze and
identify as many peptides and protein components as possible in mixture samples. Modern mass spectrometry techniques
can better achieve full-spectrum identification of proteins due to their high sensitivity, high accuracy, and high
throughput. Nowadays, shotgun proteomics is often used for protein profiling to identify samples, since the mixture
samples contain intact proteins of different molecular weights, different solubility, and different modifications.
The principle of shotgun identification is first the protein samples are digested into peptides, and separated by
high performance liquid chromatography, then the separated peptides directly enter the mass spectrometer for
analysis. Finally, the retrieval software can compare the mass spectrometry information with the corresponding data
to obtain the exact sequence of the peptide segment, and then splicing the complete sequence of various proteins.
Fig. 1. Example of shotgun
proteomics workflow. (Soufi Y, et al., 2016)
Our Services
Creative Proteomics introduces a "bottom-up" approach to protein analysis, shotgun
proteomics. Here, our highly qualified scientists utilize high-performance liquid chromatography (HPLC) techniques
combined with mass spectrometry (MS) techniques to perform full-spectrum analysis of unknown proteins.
At Creative Proteomics, shotgun protein full-spectrum analysis is the use of shotgun technology for
full-spectrum analysis of proteins, then protein identification is achieved using tandem mass spectrometry (MS/MS)
to identify peptides. Our shotgun protein profiling allows for the identification of the entire protein spectrum of
a sample, and hundreds to thousands of proteins can be identified simultaneously. The most striking feature is that
it allows for the identification and quantification of multiple proteins with minimal protein separation.
Advantages of Our Services
- High-efficiency sample separation: SDS-PAGE or SCX-HPLC can be selected according to the nature of the sample.
- High identification rate: Systematic and comprehensive study of as many proteins as possible in a tissue or
cell, identifying up to 6000 proteins.
- Wide range of application: No limitations for sample types, theoretically all biological samples are
appropriate.
- Excellent instrument performance: More accurate results can be received based on a high-resolution and accurate
mass spectrometry platform.
- Low cost: The most suitable and economy research solution can be provide for your project.
- High-quality reports: In addition to raw data, data format conversion for different needs can be provided.
- Perfect after-sales services: A certain number of original data spectra can be provided free of charge.
Applications
With our powerful mass spectrometry sequencing platform, our shotgun proteomics full-spectrum analysis has been
widely used by our customers for many different studies, including the following but not limited to:
- Whole protein expression profiling analysis.
- Protein mass spectrometry detecion.
- Plasma proteome expression analysis.
- Plant proteome expression analysis.
- Microproteome expression analysis.
- Subcellular organelle proteome expression analysis.
- Identification of interacting protein species.
Sample Requirements
- Sample type: total protein.
- Total sample amount: >500 mg (fresh tissue sample); >300 μg-500 μg (extracted protein amount).
Thanks to our powerful mass spectrometry sequencing platform, Creative Proteomics, as a first-class
protein sequencing service provider, is committed to providing global customers with protein full spectrum analysis
service to accelerate their project research journey. Our services guarantees accurate and reliable results, at
quick turnaround time! Please contact us If you are interested in them.
References
- Soufi Y, Soufi B. (2016) Mass spectrometry-based bacterial proteomics: focus on dermatologic microbial
pathogens. Front Microbiol. 7:181.
- Zhang Y, Fonslow B R, Shan B, et al. (2013) Protein analysis by shotgun/bottom-up proteomics. Chem Rev.
113(4):2343-94.
