Introduction
Glycoproteins are proteins with oligosaccharide moieties that emerge after glycosylation during post-translational or
co-translational modifications of proteins. Glycoproteins play critical roles in cellular metabolism and function,
including cell signaling, cell adhesion, the immune system, pathogenicity, occurrence and progression of metabolic
diseases, and biomarkers for diseases such as cancer. With the rapid development of protein drugs, glycoproteins
have become increasingly important in therapy, including tissue plasminogen activator for myocardial infarction and
stroke, erythropoietin for anemia, and various mono cancer therapy with cloned antibodies. However, the study of
glycoproteins is extremely difficult due to the complexity of the structure of sugar chains on proteins and their
derivatives.
In recent years, the advent of glycoproteomics based on mass spectrometry (MS) technology has provided a huge
opportunity for the analysis of glycoproteins, which has become a powerful tool to determine glycoproteins due to
its outstanding features, including sensitivity, stoichiometry, specificity and speed, which facilitates the
identification of the glycoproteins, glycosylation sites and structures of glycans.
Fig. 1. Flowchart
highlighting typical glycoproteomics work flow. (Zhu R, et al., 2018)
Our Services
Glycoproteins are widely used in biomedicine, but the complexity of sugar chains and their carbohydrates makes
glycoprotein analysis a great research challenge. With years of mass spectrometry experience and first-class
detection technology, Creative Proteomics has successfully developed a mass spectrometry-based
glycoprotein analysis platform to help customers comprehensively advance glycoprotein research. We look forward to
being your partner in your glycoprotein research journey.
At Creative Proteomics, our highly skilled staffs employ a combinatorial approach to characterize
these compounds. Throughout the service cycle, the glycoprotein of interest undergoes enzymatic digestion to release
the glycopeptide. The resulting glycopeptides are then isolated and used for various mass spectrometry studies. We
offer a variety of mass spectrometry methods for identifying a wealth of structural information about glycopeptides
by performing these analyses on glycopeptides.
To support glycoprotein engineering researchers, Creative Proteomics provides mass
spectrometry-based technologies for glycoprotein detection and glycoprotein quantification. The services we provide
include but are not limited to.
Glycoprotein structures are complex containing multiple glycan forms. We provide efficient protein structure
characterization services through different techniques for different aspects of glycan and protein structures,
including enzymatic digestion, mass spectrometry (MS), matrix-assisted laser desorption/ionization (MALDI) )-MS,
liquid chromatography systems, etc.
As a basic post-translational modification of proteins, glycosylation plays an important role in many physiological
and pathological processes, and is involved in a variety of diseases. For glycoforms in glycosylated proteins, we
provide high-quality glycan profiling services that can captures information about target glycans in a fast,
sensitive, and high-throughput manner and perform relative quantitative detection of them.
Glycosylation is of great significance in the development of biopharmaceuticals, and the analysis of glycosylation
sites is helpful for further research on glycoproteins. In addition, site-specific glycosylation analysis can
elucidate the glycosylation profile of a protein. We provide you with high-quality glycosylation site analysis
services through mass spectrometry analysis combined with other means.
Protein glycosylation modification plays a vital role in specific biological processes such as molecular recognition,
cell communication, and signal transduction. The analysis of glycosylation sites and glycoforms can help to further
elucidate the glycosylation of your glycoproteins features.
Advantages of Our Services
- Comprehensive and systematic analysis of glycoproteins in complex samples.
- Combination of multiple advanced analysis platforms.
- Low cost.
- Rapid turnaround time.
- Customized service and after-sales service.
With years of experience in mass spectrometry, Creative Proteomics provides comprehensive
glycoprotein analysis services to help customers in-depth study of glycoprotein heterogeneity and function. Our
professional research team is committed to applying the highest standards to our products and is able to provide
customers with different types of analytical data according to their specific needs. If you are interested in our
services, please contact us for more information.
References
- Zhu R, Zacharias L, Wooding K M, et al. (2016) Glycoprotein enrichment analytical techniques:
advantages and disadvantages. Methods Enzymol. 585:397-429.
- Liu H, Zhang N, Wan D, et al. (2014) Mass spectrometry-based analysis of glycoproteins and its clinical
applications in cancer biomarker discovery. Clin Proteomics. 11(1):14.
