Introduction
Antibody sequence information plays a critical role in the similarity analysis of monoclonal antibodies and the
development of biosimilar products, and is essential for antibody engineering and protein characterization in
therapeutic development. Typically, the sequence of the original gene/antibody can be obtained by searching the
published literature and patents. However, we found some discrepancies between the sequence information of mAb
products on the market and those reported in the literature/patents. Therefore, it is of great significance to
quickly obtain the complete amino acid sequence of an antibody. The most direct way to obtain complete sequence
information of monoclonal antibodies is to thoroughly and accurately sequence the whole amino acids of these
antibodies.
Fig. 1. Antibody structure. (Alexander M, et
al., 2014)
Our Services
Creative Proteomics developed an effective and efficient method for sequencing recombinant
monoclonal antibodies to determine the main amino acid sequence of monoclonal antibodies without prior knowledge of
hybridoma cell lines or DNA sequences on the basis of the mass spectrometry platform. De novo sequencing of MS/MS
spectra, using its unique internal data mining tools to ensure that target antibody sequences are extracted from
tens of thousands of MS/MS spectral data, so as to complete antibody sequencing and help researchers obtain
antibodies with 100% coverage and accuracy full length amino acid sequence information. our method can denature,
reduce, digest, and sequence a monoclonal antibody in less than an hour.
At Creative Proteomics, we were able to determine the major amino acid sequences of monoclonal
antibodies without prior knowledge of hybridoma cell lines or DNA sequences. Compared to the conventional LC-MS
peptide mapping approach, our method is the standard for extended characterization of monoclonal antibody, including
protein sequence confirmation, post-translational modification (PTM) analysis, disulfide mapping, and charge- and
sequence-based variants analysis, our fast protein sequencing method fulfill the demand for quality control testing
of an ever-increasing number of in-house produced monoclonal antibody-based therapeutic proteins to support
downstream drug development.
Here, we provide the following antibody full amino acid sequencing services.
Advantages of Our Services
- Optimized high-resolution mass spectrometry workflow and state-of-the-art antibody de novo sequencing software
ensure that the entire full amino acid antibody sequencing from the N-terminal to the C-terminal.
- A variety of enzyme digestions are used to ensure 100% coverage of the peptides.
- The platform had wide applicability to all antibody types and species.
- Low sample input: 100 µg at >90% purity.
Creative Proteomics provides professional antibody full amino acid sequencing services for global
customers. Our team has successfully sequenced thousands of antibodies, including antibody reagents, therapeutics,
and newly isolated antibodies, helping researchers decode their antibodies in a fraction of the time. Therefore,
please contact us if your antibody sequence cannot be searched in online
databases.
References
- Mao Y, Zhang L, Kleinberg A, et al. (2019) Fast protein sequencing of monoclonal antibody by real-time
digestion on emitter during nanoelectrospray. MAbs. 11(4):767-778.
