Methylation Protein Analysis Service

Introduction

Protein methylation is a post-translational modification (PTM) that regulates cellular and biological processes, including transcription, RNA processing, protein interactions, and protein dynamics. Protein methylation occurs on histones and non-histone proteins, usually on arginine and lysine, including monomethylation, symmetric/asymmetric dimethylation and trimethylation, etc.

Traditional protein methylation identification relies primarily on Edman degradation, amino acid analysis, radioisotope labeling, or antibody-based methods (including peptide and protein arrays), but these methods have certain drawbacks, including low throughput, low dynamic range, and nonspecific. In addition, the realization of these methods must rely on prior knowledge of modifications, so it is difficult to accurately identify specific modification sites and differentiate methylation states. In contrast, mass spectrometry (MS)-based techniques are the preferred method for methylation analysis due to their high throughput and wide dynamic range, including bottom-up, mid-bottom and top-down approaches.

Fig. 1. The primary types and mechanisms of protein methylation. (Dai X, et al., 2021)

Our Services

Creative Proteomics provides a high-throughput, wide dynamic range mass spectrometry platform for quantitative analysis of protein methylation.

At Creative Proteomics, our efficient and professional laboratory technicians selectively enrich methylated peptides by immunoprecipitation using antibodies specific for different methylation sites and modifications prior to MS/MS analysis, which successfully eliminates low stoichiometry caused by transient and unstable methylation modifications, thus improving sensitivity of mass mass spectrometry analysis and enableing large-scale quantitative analysis of methylated proteins.

With cutting-edge equipment and expertise in protein sequencing and mass spectrometry, we provide one-stop methylated protein quantitative analysis services. You only need to tell us the purpose of your experiment and send your samples to us, we will take care of all the follow-up matters of the project.

Our experimental workflow includes:

• Protein extraction.
• Protease cleavage.
• Methylated peptide enrichment.
• Peptide fragmentation.
• Mass spectrometry analysis.
• Bioinformatics analysis.

What Can We Offer You?

• Methylation Analysis
• Methylated Peptide Enrichment
• Methylation Site Recognition
• Methylation Quantitative Analysis
• Methylated Protein-Protein Interaction Analysis

Advantages of Our Services

• High site coverage: Methylated peptides are enriched by immunoprecipitation with specific antibodies before mass spectrometry detection.
• Wide range of analysis types: Analysis of monomethylation, dimethylation and trimethylation modifications are possible.
• High accuracy: The modified peptide segment and its modification site can be accurately determined.
• Fast turnaround time: 5-7 days for full reporting.
• Customized service: The best solution will be tailored according to your project and sample type.

Sample Requirements

1. Tissue samples: Plant tissue samples > 200 mg, blood samples > 1 mL (with EDTA for anticoagulation of plasma), serum > 0.5 mL, urine> 2 mL, animal tissue samples >1 g, cell samples > 5X10⁷ cells, microorganisms> 200 mg (dry weigh).
2. Protein samples > 1 mg.

With our powerful mass spectrometry sequencing platform, Creative Proteomics provides a one-stop shop methylation protein analysis service. Our experienced scientists work with you to develop tailor-made analytical solutions. Please feel free to contact us with any questions regarding methylation protein analysis.

References

1. Dai X, Ren T, Zhang Y, et al. (2021) Methylation multiplicity and its clinical values in cancer. Expert Rev Mol Med. 23:e2.
2. Afjehi-Sadat L, Garcia B A. (2013) Comprehending dynamic protein methylation with mass spectrometry. Curr Opin Chem Biol. 17(1):12-19.