Introduction
Compared with other traditional single-molecule detection technologies, nanopore single-molecule sensors have the
advantages of low cost and high throughput. Therefore, nanopores are widely used in some single molecules In the
detection of nucleic acid molecules, proteins, nucleic acid-protein conjugates, etc., it is even used for the
identification of single DNA bases and protein amino acids.The nanopore sequencing method uses the electrophoretic
driving force to make the single molecule to be tested pass through the nanopore one by one, and realize the
sequence determination by detecting the current change of the nanopore cross-section. When a charged biomolecule is
electrophoresed through a nanopore under the action of an applied electric field, the via current is modulated due
to its physical occupying effect and its strong interaction with the nanopore wall to obtain the charged
characteristics, shape and orientation of the molecules to be tested. At present, nanopore sequencing is mainly used
for the sequencing and identification of single-molecule proteins.
Fig. 1. Approaches to nanopore sequencing.
(Branton D, et al., 2010)
Our Services
Based on nanopore technology, Creative Proteomics have developed a method for protein fingerprinting
and combined this method with modern machine learning techniques and existing proteomic databases to enable
inexpensive and rapid proteomic quantification of single molecules. In addition, we have developed a novel method
for nanopore protein sequencing using a protein sequencing method of tagged amino acids. This method enables the
analysis of amino acid sequences of individual proteins by slowing and controlling the translocation of analytes
through nanopores.
Our Technical Advantages
- It is able to detect changes in single amino acid substitutions based on changes in ionic current readings with
Highly specific and sensitive.
- The method is able to read a single peptide string again and again to identify the molecule with essentially
100% accuracy.
- The error rate is less than one part per million.
Creative Proteomics focuses on the development and research of protein sequencing platforms. We have
a professional scientific research team who dedicated to providing advanced nanopore protein sequencing to achieve
specific resolution of natural amino acids and their post-translational modifications and accurate acquisition of
single-molecule protein sequences. If you are interested in our services, please feel free contact us. We look forward to working with you.
References
- Mazdak A B, John J K, Manuela P G, et al. (2022) Nanopore-Based Protein Identification. Journal of
the American Chemical Society. 144(6):2716-2725.
