Introduction
Acylation is an important post-translational modification of proteins, and acyl-CoA compounds act as acyl donors for
protein acylation and directly participate in protein acylation in vivo. In addition to the most thoroughly studied
acetylation, various acylation modifications have been discovered in recent years, including propionylation,
malonylation, glutarylation, succinylation, crotonylation, butyrylation, 2-hydroxyisobutyrylation, and
β-butyrylation, etc. This protein post-translational modification widely exists in prokaryotes and eukaryotes,
regulates a variety of important biological processes, and is closely related to various physiological processes
such as DNA binding, transcription, cell signal transduction, and energy metabolism. Acylation quantitative protein
analysis is a method to analyze the acylation level of biological samples in different states (physiological or
pathological) and to deeply reveal the relationships between changes of acylation modification of protein and life
activities, which is an important means to elucidate the mechanism of life activitie, screen clinical markers of
diseases and identify drug targets.
Fig. 1. Different types of
protein acylation. (Thinon E, et al., 2015)
Our Services
Creative Proteomics has launched an advanced protein acylation analysis platform with a powerful
mass spectrometry platform, and combined with nanoLC-MS/MS, on the basis of rich experience in mass spectrometry
sequencing.
At Creative Proteomics, our efficient, professional laboratory technicians employ a bottom-up
approach to analysis acylated proteins. First, the protein was digested into peptide fragments, and then the mixture
of digested peptide fragments was separated by liquid chromatography to reduce the complexity of the sample, and
finally analyzed and quantified by LC-MS/MS.
During our service cycle, we provide specific means to process acylated proteins to meet any of your needs. Before
mass spectrometry analysis, some types of acylated peptides can be enriched by high-quality acylated class-specific
antibodies bining or selective chemical labeling to achieve large-scale analysis of acylated proteins.
With cutting-edge equipment and expertise in protein sequencing and mass spectrometry, we provide one-stop acylation
quantitative protein analysis services. You only need to tell us the purpose of your experiment and send your
samples to us, we will take care of all the follow-up matters of the project.
Our analytical process includes:
- Protein extraction.
- Protein digestion.
- Specific antibody binding or selectable labeling.
- Mass spectrometry analysis.
- Bioinformatics analysis.
Here, We Can Provide But Not Limited To:
- Lysine Acylation Quantitative Protein Analysis
- Serine/Threonine Acylation Quantitative Protein Analysis
- Propionylation Quantitative Protein Analysis
- Malonylation Quantitative Protein Analysis
- Glutarylation Quantitative Protein Analysis
- Succinylation Quantitative Protein Analysis
- Crotonylation Quantitative Protein Analysis
- Palmitoylation Quantitative Protein Analysis
- Myristoylation Quantitative Protein Analysis
- Palmitoylation Site Recognition
- Protein Acylation-Protein Interactions Analysis
Advantages of Our Services
- High site coverage: Acylated peptides are enriched by selective chemical labeling or specific acylated
antibodies combining prior to mass spectrometry detection.
- Wide range of analysis types: Propionylation, succinylation, myristoylation, palmitoylation and other types of
protein acylation can be analyzed.
- High accuracy: Acylated peptides can be accurately identified on a large scale, on the basis of high-resolution
and high-throughput mass spectrometry platform.
- Experienced: Professional advices from our scientists with years of experience in protein sequencing.
- Fast turnaround: 5-7 days to provide comprehensive report.
- Customized service: Tailoring the best solution according to your project and sample type.
Sample Requirements
- Tissue samples: Plant tissue samples > 5 g, blood samples > 1 mL (with EDTA for anticoagulation of
plasma), serum > 0.5 mL, urine> 2 mL, animal tissue samples > 1 g, cell samples > 5X107
cells, microorganisms > 200 mg (dry weigh).
- Protein samples > 1mg.
Thanks to our powerful mass spectrometry sequencing platform, Creative Proteomics provides a
one-stop shop for acylation quantitative protein analysis service. Our experienced scientists work with you to
develop tailor-made analytical solutions. Please feel free to contact us
with any questions regarding acylation quantitative protein analysis.
References
- Thinon E, Hang H C. (2015) Chemical reporters for exploring protein acylation. Biochem Soc Trans.
43(2):253-61.
- Xu J Y, Xu Y, Xu Z, et al. (2018) Protein Acylation is a General Regulatory Mechanism in Biosynthetic
Pathway of Acyl-CoA-Derived Natural Products. Cell Chem Biol. 25(8):984-995.
